CELL LINES
Contributor Information
- Name Rub?n Vicente
- Institute Universitat Pompeu Fabra
- Primary citation Colomar-Carando et al. 2019. J Immunol. 202(2):441-450. PMID: 30552163.
Tool Details
- Tool name: Jurkat Zip6KO
- Tool type: Cell Line
- Parental cell line: Jurkat
- Organism: Human
- Tissue: PBMC
- Cancer type: Blood cancer
- Growth properties: Impaired Activation
- Model: Zip6KO
- Model description: CRISPR/Cas9âexpressing Jurkat cell line used to generate a Zip6KO Jurkat cell line
- CRISPR: Yes
-
Description: Cell line used to study how the absence of the Zip6 transporter affects lymphocyte physiology.
Zip6 Knockout (KO) line, Zip6 is strongly upregulated early during activiation of T lymphocytes stimulated using anti-CD3/anti-CD28 - Research area: Immunology; Cancer
- Production details: Cells were maintained in RPMI 1640 medium supplemented with 10% FBS and 1% penicillin/streptomycin. Where indicated, FBS was incubated according to the manufacturerâs instructions with Chelex 100 resin (Bio-Rad Laboratories) to generate Zn2+-free growth medium. Various amounts of ZnSO4 were added to the final medium to generate specific Zn2+ concentration conditions. Cells were grown at 37°C in a humidified 5% CO2 atmosphere.Â
- For Research Use Only
Target Details
Application Details
Handling
- Growth medium: RPMI 1640 medium
- Temperature: 37°C
- Atmosphere: 5% CO2
- Shipping conditions: Dry Ice
- Cultured in antibiotics?: 1% Penicillin-Streptomycin
Documentation
Related Tools
References
- • Colomar-Carando et al. 2019. J Immunol. 202(2):441-450. PMID: 30552163.
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