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Contributor Information

  • Name Peter Flatt and Neville McClenaghan
  • Institute Ulster University
  • Primary citation Lieber M et al. 1975. Int J Cancer 15: 741-747 PMID: 1140870

Tool Details

  • Tool name: 1.4E7 cell line
  • Tool type: Cell Line
  • Tool sub-type: Hybrid cell line
  • Parental cell line: PANC-1 cell line
  • Organism: Human
  • Tissue: Pancreas
  • Disease: Diabetes
  • Morphology: Epitheloid
  • Growth properties: Adherent
  • Model: Transgenic
  • Model description: Hybrid cell line formed by the electrofusion of a primary culture of human pancreatic islets with PANC-1, a human pancreatic ductal carcinoma cell line
  • CRISPR: No
  • Application: producing pure insulin secreting cells when stimulated
  • Description: A hybrid cell line formed by the electrofusion of a primary culture of human pancreatic islets with PANC-1, a human pancreatic ductal carcinoma cell line. 1.4E7 has been shown to be tumourigenic when transplanted into a SCID mouse host. The cell line has applications in the study of pancreatic cell biology.
  • Research area: Disease (not cancer)
  • Additional notes: Patent: Flatt P & McClenaghan N (2004) Insulin producing cell-line US patent number 20040005702 (A1). Also WO0210346 (A2).

  • For Research Use Only

Target Details

Application Details

  • Application: producing pure insulin secreting cells when stimulated

Handling

  • Format: Frozen
  • Growth medium: RPMI-1640 + 2mM Glutamine + 10% FCS
  • Temperature: 37°C
  • Atmosphere: 5% CO2
  • Storage conditions: Liquid Nitrogen
  • Shipping conditions: Dry Ice
  • Initial handling information: Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-4 x 104 cells/cmÂ? using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37°C. Population doubling approx 26hrs. At confluence 105 cells/cmÂ? can be expected.
  • STR profile: STR-PCR Data: Amelogenin: X CSF1PO: 10,12 D13S317: 11 D16S539: 11 D5S818: 11,13 D7S820: 8 THO1: 7,8 TPOX: 8,11 vWA: 15
  • Mycoplasma free: Yes

Documentation

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References

  • •  McCluskey JT et al. 2011. J Biol Chem. Apr 22. PMID: 21515691., Guo-Parke H et al. 2012. J Endocrinol. 214(3):257-65. PMID: 22685334