CELL LINES
Contributor Information
- Name Ghanem Elias, Ahmad Najem
- Institute Universit? libre de Bruxelles
Tool Details
- Tool name: MM057-N375S cell line
- Tool type: Cell Line
- Parental cell line: Ham's F10 melanoma culture medium (HMCM)
- Organism: Human
- Donor: Derived from a lymph node metastasis
- Tissue: Lymphatic
- Gender: Female
- Cancer type: Skin cancer
- Disease: Cancer
- Growth properties: Adherent
- Model: MET: N375S
- Model description: This cell line displays an intermediate phenotype ( melanocytic state with increased mesenchymal-like properties)
- CRISPR: No
- Application: High-throughput screening/Drug testing - 3D cell cultures
- Description: Emelanotic cell line with intermediate sensitivity to MEKi
- Research area: Cancer
- For Research Use Only
Target Details
Application Details
- Application: High-throughput screening/Drug testing - 3D cell cultures
Handling
- Format: Frozen
- Growth medium: Hamâs F10 supplemented with 10% of fetal calf serum (FCS), with L-glutamine (1mM), penicillin (100 IU/ml), and streptomycin (100 Â?g/ml). Renewal 2 to 3 times per week
- Temperature: 37°C
- Atmosphere: 5% CO2
- Storage conditions: Liquid Nitrogen
- Shipping conditions: Dry Ice
- Initial handling information: The frozen culture should be stored in liquid nitrogen upon arrival or thaw the vial and initiate the culture as soon as possible upon receipt
- Cultured in antibiotics?: Yes
- STR profile: Cell Line Authentication was performed using STR profiling with AmpFLSTRâ? Identifilerâ? PCR Amplification Kit (Thermo Fisher Scientific). 16 independent PCR-systems (D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, AMEL, D5S818, FGA, D19S433, vWA, TPOX, and D18S51) were investigated and analyzed (Eurofins Genomics, Germany). The STR profile will be provided
- Biosafety level: BSL-2
- Subculture routine: Split sub-confluent cultures (70-80%) 1:2 to 1:5
Documentation
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