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MM029 cell line

Cat. #161610

Tool name: MM029 cell line
Tool type: Cell Line
Parental cell line: Ham's F10 melanoma culture medium (HMCM)
Organism: Human
Donor: Derived from a lymph node metastasis of a female with superficial spreading melanoma (SSM)
Tissue: Lymphatic
Gender: Female
Cancer type: Skin cancer
Disease: Cancer
Growth properties: Adherent
Model: BRAF: V600K
Model description: This cell line displays an invasive/Mesenchymal_like phenotype
CRISPR: No
Application: High-throughput screening/Drug testing - 3D cell cultures
Description: Cell Pigmentation:Unpigmented cell line. Drug response: Intrinsic resistance to BRAFi
Research area: Cancer

Format: Frozen
Growth medium: Hamâs F10 supplemented with 10% of fetal calf serum (FCS), with L-glutamine (1mM), penicillin (100 IU/ml), and streptomycin (100 Â?g/ml). Renewal 2 to 3 times per week
Temperature: 37Â°C
Atmosphere: 5% CO2
Storage conditions: Liquid Nitrogen
Shipping conditions: Dry Ice
Initial handling information: The frozen culture should be stored in liquid nitrogen upon arrival or thaw the vial and initiate the culture as soon as possible upon receipt
Cultured in antibiotics?: Yes
STR profile: Cell Line Authentication was performed using STR profiling with AmpFLSTRâ? Identifilerâ? PCR Amplification Kit (Thermo Fisher Scientific). 16 independent PCR-systems (D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, AMEL, D5S818, FGA, D19S433, vWA, TPOX, and D18S51) were investigated and analyzed (Eurofins Genomics, Germany). The STR profile will be provided
Biosafety level: BSL-2
Subculture routine: Split sub-confluent cultures (70-80%) 1:2

Quantity:

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