CELL LINES
Contributor Information
- Name William G Watson
- Institute University College Dublin
- Primary citation O'Neill et al. Mol Cancer. 2011 Oct 7;10:126. PMID: 21982118
Tool Details
- Tool name: PC-3 Ag cell line
- Alternate names: PC-3 age matched cell line
- Tool type: Cell Line
- Organism: Human
- Tissue: Pancreas
- Cancer type: Pancreatic cancer
- Disease: Cancer
- Growth properties: Suspension
- Model: Mutant
- CRISPR: No
- Products or characteristics of interest: Age matched cell line
- Application: Mechanisms of resistance to Docetaxe in pancreatic cancer cells
- Description: Docetaxel resistant sub-lines were generated in the androgen-independent (PC-3, DU-145) and sensitive (22RV1) cell lines. One of the main mechanisms of resistance to Docetaxel is the over expression of P-gp which would reduce intracellular concentrations of the drug through increased efflux. Chemoresistant PC-3 cells do not over express P-gp. We demonstrated that the resistance in the PC-3 sublines was independent of P-gp over expression as there were no detectable levels and no effects of the P-gp inhibitor Elacridar on their resistance
- Research area: Cancer
- Production details: PC-3 resistant sub-lines were generated by initially treating with Docetaxel (Sigma) at 4 nM and 8 nM (suspended in dimethyl sulfoxide (DMSO) (Fluke)) in 75 cm2 flasks for 48 hours. After treatment, the surviving cells were re-seeded into new flasks and allowed to recover for 2-3 weeks. After 5 (at 8 nM) and 7 (at 4 nM) treatments, the dose of docetaxel was increased from 4 nM and 8 nM to 8 nM and 12 nM respectively. The cells underwent a total of 18 treatment cycles at 8 nM and 12 treatment cycles at 12 nM, (cell passage numbers 21-55). Following each treatment they were allowed to fully recover before assessing their resistance to docetaxel and any experimental work.
- For Research Use Only
Target Details
Application Details
- Application: Mechanisms of resistance to Docetaxe in pancreatic cancer cells
Handling
- Format: Frozen
- Growth medium: RPMI-1640 medium supplemented with 10% Fetal Bovine Serum (FBS), 50 U/ml penicillin/50 ?g/ml streptomycin and 2 mM L-glutamine
- Storage conditions: Liquid Nitrogen
- Shipping conditions: Dry Ice
Documentation
Related Tools
References
- • O'Neill et al. Mol Cancer. 2011 Oct 7
- • 10:126. PMID: 21982118
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