CELL LINES
Contributor Information
- Name Ji?? Neu?il
- Institute Institute of Biotechnology of the Academy of Sciences of the Czech Republic
- Primary citation Ezrova et al. Oncogene. 2021 Apr, 40(14):2539-2552. PMID: 33686239
Tool Details
- Tool name: PaTu 8902 SMAD4 KOrec reconstituted cell line
- Alternate names: PaTu 8902 SMAD4-deficient cells, reconstituted wt SMAD4
- Tool type: Cell line
- Parental cell line: PaTu 8902
- Organism: Human
- Tissue: Pancreas
- Gender: Female
- Cancer type: Pancreatic cancer
- Disease: Cancer
- Morphology: Epithelial cells
- Growth properties: Adherent
- Model: Transgeneic
- Model description: SMAD4 knock-out cells, wild-type SMAD4 reconstituted using lentiviral transduction (pCDH-CMV vector)
- CRISPR: Yes
- Conditional: No
- Products or characteristics of interest: Stable expression of SMAD4
- Description: SMAD4 is an important tumor suppressor involved in transforming growth factor ? (TGF?) signaling, and associated with altered mitochondrial activity. The resistance of SMAD4-deficient cells is mediated by increased mitophagic flux driven by MAPK/ERK signaling, whereas TGF?-induced resistance is autophagy-independent and linked to epithelial-to-mesenchymal transition (EMT). Mitochondria-targeted tamoxifen, a complex I inhibitor under clinical trial, overcomes resistance mediated by SMAD4-deficiency or TGF? signaling. Our data point to differential mechanisms underlying the resistance to treatment in PDAC arising from TGF? signaling and SMAD4 loss, respectively.
- Research area: Cancer
- For Research Use Only
Target Details
Application Details
Handling
- Growth medium: DMEM+10% FBS
- Temperature: 37C
- Atmosphere: 5% CO2
- Storage medium: Complete medium + 10% DMSO
- Storage conditions: Liquid Nitrogen
- Shipping conditions: Dry Ice
- Cultured in antibiotics?: Yes
- Mycoplasma free: Yes
- Biosafety level: 1
- Subculture routine: Trypsin-EDTA
Documentation
References
- • Ezrova et al. Oncogene. 2021 Apr, 40(14):2539-2552. PMID: 33686240
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