CELL LINES
Contributor Information
- Name Medical-Industrial Translational Research Center
- Institute Fukushima Medical University
Tool Details
- Tool name: MCF10A-TP53m-10 cell line
- Alternate names: 06M04011
- Clone: TP53-a_type-10
- Tool type: Cell Lines
- Tool sub-type: Continuous
- Parental cell line: MCF10A, a non-tumorigenic cell line from human mammarygland epithelium
- Organism: Human
- Tissue: Mammarygland epithelium
- Cancer type: Non-tumorigenic
- Morphology: Epithelial
- Growth properties: Adherent
- Model: Mutant
- Model description: Type: Type-10; Type mutation: R213*; CDS mutation: c.637C>T; AA mutation: p.R213*; COSMIC Mutation ID: COSM10654
- Application: Functional analysis of mutated genes, Drug screening
- Description: Non-tumorigenic immortalized breast epithelial cell stably overexpressing mutant cancer-related genes. TP53 (tumor protein p53). TP53 is a cancer suppressor gene responding to stimuli and damage of DNA that serves to protect normal cells by repairing DNA and regulating proliferation. Its mutations are found in 50% of all cancers. It is said that the mutated TP53 gene loses its original function to protect cells and cause the accumulation of gene mutations, apoptosis avoidance and abnormal proliferation of cells. Most of the mutations are concentrated in the DNA-binding region and mutations of R248, R273, R175 and R249 are representative mutations. All of these mutations are known to impact the transformation ability in cooperation with activated RAS.
- Research area: Cancer; Cell biology
- For Research Use Only
Target Details
- Target: Tumor protein p53 [TP53]
- Target background: Gene ID: 7157; References: DNA (mRNA): NM_000546.5; Protein: NP_000537.3
Application Details
- Application: Functional analysis of mutated genes, Drug screening
Handling
- Format: Frozen
- Growth medium: DMEM/Ham's F-12 supplemented with 5% heat-inactivated horse serum, 10 Â?g/ml insulin (human, recombinant), 5 Â?M forskolin, 0.5 Â?g/ml hydrocortisone, 20 ng/ml EGF (human, recombinant), 100 U/ml penicillin, and 100 Â?g/ml streptomycin
- Temperature: 37?C
- Atmosphere: Humidified incubator with 5%Â CO2
- Storage medium: CELLBANKER 2 (Zenogen pharma)
- Storage conditions: Liquid Nitrogen
- Shipping conditions: Dry ice
- Characterisation tests: Comprehensive gene expression analysis; Identification of cell proliferation and cellular form; Drug sensitivity tests
- Initial handling information: Thaw the vial at 37 °C in a water bath?then seed cells into a T75 flask. Culture in a humidified Incubator at 37 ? with 5% CO2 in air. Culture medium should be changed every 2 to 3 days.
- Cultured in antibiotics?: 100 U/ml Penicillin, and 100 Â?g/ml Streptomycin
- Mycoplasma free: Yes
- Biosafety level: 1
Documentation
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