CELL LINES
Contributor Information
- Name Medical-Industrial Translational Research Center
- Institute Fukushima Medical University
Tool Details
- Tool name: MCF10A-PIK3CAm-3 cell line
- Alternate names: 06M03004
- Clone: PIK3CA_type-03
- Tool type: Cell Lines
- Tool sub-type: Continuous
- Parental cell line: MCF10A, a non-tumorigenic cell line from human mammarygland epithelium
- Organism: Human
- Tissue: Mammarygland epithelium
- Cancer type: Non-tumorigenic
- Morphology: Epithelial
- Growth properties: Adherent
- Model: Mutant
- Model description: Type: Type-03; Type mutation: E542K; CDS mutation: c.1624G>A; AA mutation: p.E542K; COSMIC Mutation ID: COSM760
- Application: Functional analysis of mutated genes, Drug screening
- Description: Non-tumorigenic immortalized breast epithelial cell stably overexpressing mutant cancer-related genes. PIK3CA (phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit alpha). PIK3CA belongs to the gene family of phosphatidylinositol-3 kinase (PI3K), important regulating factor of signaling cascade, is known to cause various biological activities such as cell differentiation, proliferation, metabolism, cell migration and reconstruction of the cell skeleton through downstream molecules. Mutations of the PIK3CA gene are frequently found in glioblastoma, and colorectal, stomach and breast cancer. Most of the mutations are known to be concentrated in the 2 region called hot spot (Exon9 and Exon20) in the helix or the kinase domain of PI3K.
- Research area: Cancer; Cell biology
- For Research Use Only
Target Details
- Target: Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha [PIK3CA]
- Target background: Gene ID: 5290; References: DNA (mRNA): NM_006218.2; Protein: NP_006209.2
Application Details
- Application: Functional analysis of mutated genes, Drug screening
Handling
- Format: Frozen
- Growth medium: DMEM/Ham's F-12 supplemented with 5% heat-inactivated horse serum, 10 Â?g/ml insulin (human, recombinant), 5 Â?M forskolin, 0.5 Â?g/ml hydrocortisone, 20 ng/ml EGF (human, recombinant), 100 U/ml penicillin, and 100 Â?g/ml streptomycin
- Temperature: 37?C
- Atmosphere: Humidified incubator with 5%Â CO2
- Storage medium: CELLBANKER 2 (Zenogen pharma)
- Storage conditions: Liquid Nitrogen
- Shipping conditions: Dry ice
- Characterisation tests: Comprehensive gene expression analysis; Identification of cell proliferation and cellular form; Drug sensitivity tests
- Initial handling information: Thaw the vial at 37 °C in a water bath?then seed cells into a T75 flask. Culture in a humidified Incubator at 37 ? with 5% CO2 in air. Culture medium should be changed every 2 to 3 days.
- Cultured in antibiotics?: 100 U/ml Penicillin, and 100 Â?g/ml Streptomycin
- Mycoplasma free: Yes
- Biosafety level: 1
Documentation
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