CELL LINES
Contributor Information
- Name Medical-Industrial Translational Research Center
- Institute Fukushima Medical University
Tool Details
- Tool name: MCF10A-NRASm-3 cell line
- Alternate names: 06M00064
- Clone: NRAS_type-03
- Tool type: Cell Lines
- Tool sub-type: Continuous
- Parental cell line: MCF10A, a non-tumorigenic cell line from human mammarygland epithelium
- Organism: Human
- Tissue: Mammarygland epithelium
- Cancer type: Non-tumorigenic
- Morphology: Epithelial
- Growth properties: Adherent
- Model: Mutant
- Model description: Type: Type-03; Type mutation: G12D; CDS mutation: c.35G>A; AA mutation: p.G12D; COSMIC Mutation ID: COSM564
- Application: Functional analysis of mutated genes, Drug screening
- Description: Non-tumorigenic immortalized breast epithelial cell stably overexpressing mutant cancer-related genes. NRAS (neuroblastoma RAS viral (v-ras) oncogene homolog). A cancer gene that belongs to the low-molecule GTPase called the RAS family. It is thought to play an essential role in the MAPK signaling pathway like KRAS, a representative of the RAS family. While the mutation of KRAS is frequently found in bowel cancer (approx. 30%), the frequency of NRAS's mutation in bowel cancer is approximately 4% and it is known to be found more often in skin tumors (approx. 19%) and blood tumors such as leukemia and myelodysplastic syndrome (approx. 12%). Mutation of Q61 and G12 are known as somatic cell mutations that are often found in cancer.
- Research area: Cancer; Cell biology
- For Research Use Only
Target Details
- Target: Neuroblastoma RAS viral oncogene homolog [NRAS]
- Target background: Gene ID: 4893; References: DNA (mRNA): NM_002524.4; Protein: NP_002515.1
Application Details
- Application: Functional analysis of mutated genes, Drug screening
Handling
- Format: Frozen
- Growth medium: DMEM/Ham's F-12 supplemented with 5% heat-inactivated horse serum, 10 Â?g/ml insulin (human, recombinant), 5 Â?M forskolin, 0.5 Â?g/ml hydrocortisone, 20 ng/ml EGF (human, recombinant), 100 U/ml penicillin, and 100 Â?g/ml streptomycin
- Temperature: 37?C
- Atmosphere: Humidified incubator with 5%Â CO2
- Storage medium: CELLBANKER 2 (Zenogen pharma)
- Storage conditions: Liquid Nitrogen
- Shipping conditions: Dry ice
- Characterisation tests: Comprehensive gene expression analysis; Identification of cell proliferation and cellular form; Drug sensitivity tests
- Initial handling information: Thaw the vial at 37 °C in a water bath?then seed cells into a T75 flask. Culture in a humidified Incubator at 37 ? with 5% CO2 in air. Culture medium should be changed every 2 to 3 days.
- Cultured in antibiotics?: 100 U/ml Penicillin, and 100 Â?g/ml Streptomycin
- Mycoplasma free: Yes
- Biosafety level: 1
Documentation
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