CELL LINES
Contributor Information
- Name T.R. Santhosh Kumar
- Institute Rajiv Gandhi Centre for Biotechnology
- Primary citation Lupitha SS, et.al. PMID: 32109534.
Tool Details
- Tool name: U-251/EGFP_ LC3 - Mito DsRed
- Tool type: Cell Lines
- Parental cell line: U-251MG
- Organism: Human
- Tissue: Brain
- Gender: Male
- Disease: Cancer
- Morphology: Epithelial
- Growth properties: Adherent
- Model: Transgenic
- CRISPR: No
- Conditional: No
- Description: Astrocytoma cell line U-251 stably expressing EGFP-LC3 and Mito DsRed for use as a mitophagy sensor.
- Research area: Cancer; Disease (not cancer); Metabolism
- For Research Use Only
Target Details
Application Details
Handling
- Growth medium: DMEM with 2mM L-Glutamine and 10% FBS (Heat inactivated).
- Temperature: 37C
- Atmosphere: 5% CO2
- Storage medium: Growth medium + 10% DMSO
- Storage conditions: Liquid Nitrogen
- Shipping conditions: Dry Ice
- Initial handling information: Resuscitation: Rapidly thaw the frozen ampoule in a water bath at 37?°C for 1-2 minutes. Transfer the contents to a centrifuge tube and slowly add 5-10ml of pre-warmed growth media. Remove a sample for counting. Centrifuge at 100-150g for 2-3 minutes to pellet cells (a centrifugation step to remove the cryoprotectant is essential. Seed at the recommend density, e.g. greater than 10,000 cells per cm2.
- Cultured in antibiotics?: No
- Subculture routine: Subculture non-confluent cultures using trypsin/EDTA and 1:3 to 1:4, i.e. seeding at 1-4 x10,000 cells/cm? .
Documentation
Related Tools
References
- • Lupitha SS, et.al. PMID: 32109534.
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