CELL LINES
Contributor Information
- Name Amy Chadwick
- Institute University of Liverpool, Molecular and Clinical Pharmacology
Tool Details
- Tool name: UOL-Hep-CYB8 cell line
- Tool type: Cell Lines
- Parental cell line: HepG2
- Organism: Human
- Tissue: Liver
- Disease: Mitochondrial Subhaplogroup : J2b1g
- Growth properties: Adherent
- Model description: Cybrid Cell Line
- Description: This cell line consists of HepG2 cybrid cell lines negative for cellular mitochondrial DNA, fused with different platelet partners (platelets contain only mitochondrial DNA and no nuclear DNA). Each cybrid cell line has a combination of SNPs in mitochondrial DNA, relevant to a variety of diseases. Cybrid cell line panel has potential use for drug testing
- Research area: Drug development
- Additional notes: Parental cell line was purchased from ECACC
- For Research Use Only
Target Details
Application Details
- Application notes: SNPs characteristic of assigned haplogroup : 73G 150T 152C 263G 489C 750G 1438G 4769G 5633T 7028T 8860G 9872G 10172A 10398G 11251G 11719A 12612G 13708A 14766T 15257A 15326G 15452A 15812A 16069T 16126C 16193T Additional SNPs : 2789T 13821T
Handling
- Growth medium: DMEM high-glucose medium (glucose; 25?mM) supplemented with FBS (10 % v/v), L-glutamine (4?mM), sodium pyruvate (1?mM), and HEPES (1?mM)
- Temperature: 37C
- Atmosphere: 5% CO2
- Subculture routine: Cells are subcultured weekly (1:12) via trypsinisation.
Documentation
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