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Contributor Information

  • Name Thomas Carey
  • Institute University of Michigan
  • Primary citation Owen et.al. PMCID: PMC4153472

Tool Details

  • Tool name: UMSCC-103 cell line
  • Tool type: Cell Lines
  • Organism: Human
  • Donor: Cell line deived from a 26 year-old female with a T4N2bM0 grade moderately differentiatied primary lesion of the lateral tongue. Therapies: hemiglassectomy and bilateral neck resections. Multiple lymph node containing metastases and recurrance at primary site. Therapies included post-operative radiation and chemotherapy (taxol and platinum)
  • Tissue: Oral Cavity
  • Gender: Female
  • Cancer type: Skin cancer
  • Disease: Cancer
  • Morphology: Epithelial
  • CRISPR: No
  • Research area: Cancer
  • Production details: Cell line derived by explant culture and enzymatic digestion of surgically removed tissue followed by cell sorting with flow cytometry using stem cell markers

  • For Research Use Only

Target Details

Application Details

Handling

  • Growth medium: Complete Dulbecco?‚Â’s Modified Eagle?‚Â’s Medium (cDMEM) containing 2 mM L-glutamine, 1% nonessential amino acids, 1% Penicillin-Streptomycin (optional) and 10% fetal bovine serum
  • Temperature: 37C
  • Atmosphere: 5% CO2
  • Storage medium: 90% Fetal calf serum + 10% DMSO; 10% DMEM +20% Fetal Calf Serum + 10% DMSO.
  • Storage conditions: Liquid Nitrogen
  • Shipping conditions: Dry Ice
  • Initial handling information: Resuscitation: Rapidly thaw the frozen ampoule in a water bath at 37?‚°C for 1-2 minutes. Transfer the contents to a centrifuge tube and slowly add 5-10ml of pre-warmed growth media. Remove a sample for counting. Centrifuge at 100-150g for 2-3 minutes to pellet cells (a centrifugation step to remove the cryoprotectant is essential. Seed at the recommend density, e.g. greater than 10,000 cells per cm2.
  • Cultured in antibiotics?: 1% Penicillin-Streptomycin
  • Biosafety level: 1
  • Subculture routine: Subculture non-confluent cultures using trypsin/EDTA and 1:3 to 1:4, i.e. seeding at 1-4 x10,000 cells/cm?‹Â› .

Documentation

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References

  • •  Owen et.al. PMCID: PMC4153472