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Contributor Information

  • Name Thomas Carey
  • Institute University of Michigan
  • Primary citation Carey et al. 1983. Otolaryngol Head Neck Surg. Oct;91(5):482-91. PMID: 6417595

Tool Details

  • Tool name: UMSCC-14C cell line
  • Tool type: Cell Lines
  • Organism: Human
  • Donor: Cell line de rived from 58 year old female with a poorly differentiated T1N0M0 grade tumour at floor of the mouth. Surgery, radiation therapy given followed by surgery again andchemotherapy. See (UM-SCC-14A and 14B)
  • Tissue: Oral cavity
  • Gender: Female
  • Cancer type: Head and neck cancer; Skin cancer
  • Disease: Cancer
  • Morphology: Epithelial
  • Growth properties: Adherent culture
  • CRISPR: No
  • Description: Head and neck squamous cell carcinomas (HNSCCs) are the sixth most common cancer worldwide and consist of malignant tumors of the oral cavity, oropharynx, hypopharynx, and larynx. They are known to arise due to a variety of etiologic factors including tobacco-exposure, alcohol consumption and high risk human papilloma virus (HPV) infection. Clinical outcomes and treatments vary by anatomic site with 5-year survival rates ranging from 40-80% depending on stage, subsite, and HPV status. It is important to build models representing for each specific HNSCC subsite in order to model differences between subsites. Cancer cell lines provide an invaluable research tools to study the diversity of head and neck cancers. These cell lines provide valuable tools for in vitro studies to investigate key regulatory pathways, determine malignant drivers, and discriminate potential therapeutic targets in genetically characterized models.
  • Research area: Cancer
  • Production details: Cell line derived by explant culture and partial trypsinisation of surgically removed tissue

  • For Research Use Only

Target Details

Application Details

Handling

  • Format: Frozen
  • Growth medium: Complete Dulbecco?‚Â’s Modified Eagle?‚Â’s Medium (cDMEM) containing 2 mM L-glutamine, 1% nonessential amino acids, 1% Penicillin-Streptomycin (optional) and 10% fetal bovine serum
  • Temperature: 37C
  • Atmosphere: 5% CO2
  • Storage medium: 90% Fetal calf serum + 10% DMSO; 10% DMEM +20% Fetal Calf Serum + 10% DMSO.
  • Storage conditions: Liquid Nitrogen
  • Initial handling information: Resuscitation: Rapidly thaw the frozen ampoule in a water bath at 37?‚°C for 1-2 minutes. Transfer the contents to a centrifuge tube and slowly add 5-10ml of pre-warmed growth media. Remove a sample for counting. Centrifuge at 100-150g for 2-3 minutes to pellet cells (a centrifugation step to remove the cryoprotectant is essential. Seed at the recommend density, e.g. greater than 10,000 cells per cm2.
  • Cultured in antibiotics?: 1% Penicillin-Streptomycin
  • STR profile: PMID: 19760794
  • Biosafety level: 1
  • Subculture routine: Subculture non-confluent cultures using trypsin/EDTA and 1:3 to 1:4, i.e. seeding at 1-4 x10,000 cells/cm?‹Â› .

Documentation

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References

  • •  Alley M.C, et.al. PMID: 3335022
  • •  Koldovsky P, et.al. PMID: 8679156
  • •  Frank C.J, et.al. PMID: 9041179
  • •  Welters M.J.P, et.al. PMID: 9139877
  • •  Backus H.H.J, et.al. PMID: 10956384
  • •  Takebayashi S, et.al. PMID: 15287027
  • •  Raschke S, et.al. PMID: 15495191
  • •  Lin C.J, et.al. PMID: 17312569
  • •  Hoffmann T.K, et.al. PMID: 18487078
  • •  Brenner J.C, et.al. PMID: 19760794
  • •  Schuler P.J, et.al. PMID: 20947470
  • •  Martens-de Kemp S.R., Dalm S.U. PMID: 23613873
  • •  Stoepker C, et.al. PMID: 26122845
  • •  van Harten A.M, et.al. PMID: 31541927