CELL LINES
Contributor Information
- Name Saiyed Taslimarif ; Patel Paresh Brijalal ; Mukhopadhyaya Rabindranath
- Institute The Centre for Cellular and Molecular Platforms C-CAMP
Tool Details
- Tool name: Recombinant CHO-K1 cell line with integrated Technology for enhanced production of Human Erythropoietin protein, in Adherent Cells
- Alternate names: haematopoietin, or haemopoietin
- Tool type: Cell Lines
- Parental cell line: CHO-K1
- Organism: Hamster
- Donor: The CHO-K1 cells were transduced with the two different lentiviral vectors for stable constitutive expression of 1) Human EPO gene and 2) a ShRNA targeting a specific protease that can cleave the EPO protein. G418 and Puromycin were used for selection of stable clones. The EPO is expressed without any affinity tag and is actively secreted in the culture supernatant.
- Tissue: Ovary
- Disease: Therapeutics; Haematopoiesis; Anemia
- Conditional: No
- Research area: Drug development
- Production details: The CHO-K1 cells were transduced with the two different lentiviral vectors for stable constitutive expression of 1) Human EPO gene and 2) a ShRNA targeting a specific protease that can cleave the EPO protein. G418 and Puromycin were used for selection of stable clones. The EPO is expressed without any affinity tag and is actively secreted in the culture supernatant.
- For Research Use Only
Target Details
- Target: Erythropoietin (EPO)
Application Details
Handling
- Growth medium: F12 media supplemented with 10% FBS and 1% of 5000U/ml Penstrep (penicillin G and streptomycin) antibiotic. The cells are grown in a CO2 incubator at 37?°C with 5% CO2.
- Temperature: 37C
- Atmosphere: 5% CO2
Documentation
Related Tools
References
- • A VECTOR, A HOST CELL AND A METHOD THEREOF (PCT/IB2019/052213)
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