CELL LINES
Contributor Information
- Institute The University of Minnesota, Twin Cities
Tool Details
- Tool name: SARS-CoV-2 RBD fragment cell line
- Alternate names: Recombinant SARS-CoV-2 RBD
- Tool type: Cell Lines
- Parental cell line: HEK293F
- Organism: Human
- Donor: To constitutively express SARS-CoV-2 RBD, HEK293F cell line was genetically modified in the lab using the lentivirus system. More specifically, the SARS-CoV-2 RBD gene was inserted into the genome of HEK293F cells using lentiviruses, and the stably transfected cells constitutively secrete SARS-CoV-2 RBD into the cell medium at high levels. The RBD fragment has 8X his tags for subsequent purification.
- Cancer type: Antigen; Protein fragment; Receptor binding domain
- Disease: Coronavirus; COVID-19; SARS-CoV-2
- Description: The SAR-CoV-2 RBD fragment expressed by this cell line has been successfully used to make ELISA assays for detection of coronavirus infection in human patients.
- Research area: Drug development; Immunology; Biochemistry; Microbiology
- Production details: To constitutively express SARS-CoV-2 RBD, HEK293F cell line was genetically modified in the lab using the lentivirus system. More specifically, the SARS-CoV-2 RBD gene was inserted into the genome of HEK293F cells using lentiviruses, and the stably transfected cells constitutively secrete SARS-CoV-2 RBD into the cell medium at high levels. The RBD fragment has 8X his tags for subsequent purification.
- For Research Use Only
Target Details
- Target: Antigen, protein fragment, receptor binding domain
Application Details
Handling
- Growth medium: The cells are cultured in FreeStyle 293 Expression Medium from Thermo Fisher.
Documentation
Related Tools
References
- • Shang et al. 2020. Nature. 581(7807):221-224. PMID: 32225175.
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