CELL LINES
Contributor Information
- Name Maria Carmo-Fonseca
- Institute Instituto de Medicina Molecular Jo?Âo Lobo Antunes
Tool Details
- Tool name: iPSC healthy control for neuronal differentiation (F002.1A.13) cell line
- Tool type: Cell Lines
- Parental cell line: Human fibroblasts
- Organism: Human
- Tissue: Fibroblast
- Disease: Cerebellar diseases
- Growth properties: Healthy control
- Model: Stem Cells
- Conditional: No
- Description: Healthy control ÄË?Â? optimized for cerebellar neurons differentiation. Generation of electrophysiologically active GABAergic and glutamatergic cerebellar neurons in long-term cultures without co-culturing with other cell types. Relevant model for drug screening and for study specific pathways involved with the development of cerebellar diseases such as ataxias, a group of disorders that affect many children and adults worldwide.
- Research area: Neurobiology; Genetics; Stem cell biology
- Production details: Reprogramming of human induced pluripotent stem cells from a skin biopsy of a female healthy donor.
- For Research Use Only
Target Details
- Target: Healthy control
Application Details
Handling
- Format: Frozen
- Growth medium: Cells are cultured on MatrigelTM (Corning)-coated plates with mTeSRTM1 Medium (StemCell Technologies). Medium is changed daily. Cells are passaged every 3ÄË?Â?Â4 days (when the colonies covered approximately 85% of the surface area of the culture dish) using 0.5 mM EDTA dissociation buffer (Life Technologies). Cells are incubated at 37 ÄË?ÂÄÂC in a humidified 5% CO2 incubator.
- Shipping conditions: Dry ice
Documentation
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