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Contributor Information

  • Name Sergio F de Almeida
  • Institute Instituto de Medicina Molecular Joo Lobo Antunes

Tool Details

  • Tool name: EX2 Flp reporter cell line
  • Tool type: Cell Lines
  • Tool sub-type: Continuous
  • Parental cell line: Flp-In T-REx 293 cell line
  • Organism: Human
  • Tissue: Embryonic kidney
  • Model: Reporter
  • Conditional: No
  • Description: Derived from embryonic kidney cells
  • Research area: Genetics; Cell biology; Genetics
  • Production details: The pOG44 Flp-recombinase expression vector was co-transfected with the EX2 reporter construct into Flp-Inâ?‚„?‹Â˜ T-RExâ?‚„?‹Â˜ 293 cells

  • For Research Use Only

Target Details

Application Details

Handling

  • Format: Frozen
  • Growth medium: Maintain under selection with 200 ?g/ml Hygromycin B (Roche) and 15 ?g/ml Blasticidin (InvivoGen) at 37?‚°C in a humidified atmosphere with 5% CO2.
  • Shipping conditions: Dry ice

Documentation

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References

  • •  Vtor et al. 2019. Sci Adv. 5(1):eaau1249. PMID: 30662944.