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Contributor Information

  • Name Martin Clynes
  • Institute Dublin City University

Tool Details

  • Tool name: DLKP-pHA MDR 1/A cell line
  • Tool type: Cell Lines
  • Tool sub-type: Continuous
  • Parental cell line: DLKP
  • Organism: Human
  • Tissue: Lymph Node
  • Cancer type: Lung cancer
  • Disease: Cancer
  • Growth properties: monolayer, slow-growing
  • Conditional description: fold resistance comp. toÂ?‚ DLKP parent: 15-fold Adriamycin; 12.2 Vincristin; 13.6 VP-16 (Etoposide).
  • Description: heterogeneous lung carcinoma population. Epithelial model
  • Research area: Cancer; Drug development; Cell biology
  • Production details: DLKPÂ?‚ transfected with a pHaMDR1/A plasmid (full cDNA for human MDR1 gene) and treated with 50mg/ml adriamycin

  • For Research Use Only

Target Details

Application Details

Handling

  • Format: Frozen
  • Growth medium: DMEM/Ham's F12, 5% FCS supplement with 50ng/ml adriamycin at least once/week. Passage 2-3 time weekly, spin after trypsinisation. Freezing: 5% DMSO, freeze at 2x10(6) cells/ml, spin down after reviving
  • Storage conditions: Liquid Nitrogen
  • Shipping conditions: Dry ice
  • Mycoplasma free: Yes
  • Biosafety level: 1

Documentation

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