CELL LINES
Contributor Information
- Name Andrew Crowe
- Institute Curtin University
Tool Details
- Tool name: CLEFF4 cell line
- Tool type: Cell Lines
- Parental cell line: Caco-2
- Organism: Human
- Tissue: Colon
- Model: Mutant
- Description: Pharmaceutical drug development requires drug targets to be characterised against efflux transporters. An example is P-glycoprotein (P-gp) which prevents the uptake and trans-cellular transport of hundreds of drug groups. The standard model is to use Caco-2 cells as these can be induced to express high concentrations of P-gp. Said tight junction model can approximate the role of P-gp at the barrier sites in the body including the gastrointestinal tract and also the blood-brain-barrier. There are however limitations to the Caco-2 cell line, including taking 24 days to develop the characteristics required for P-gp-mediated efflux modelling. Further, other efflux proteins including BCRP and MRP2 are often present in Caco-2 cells, which add extra variables when characterising specific P-gp mediated effects. The CLEFF4 cell line is ready to use in just 5 days and has a 3-fold higher expression of P-gp cells at 5-7 days compared to Caco-2 cells at day 21, expressing no more MRP2 and less BCRP than the parent Caco-2 cells. This is a faster, more specific model for P-gp assays offering cost and time savings and which has reduced medium requirement (one can use standard not accelerated media).
- Research area: Drug development
- For Research Use Only
Target Details
- Target: CLEFF4
Application Details
Handling
- Format: Frozen
- Shipping conditions: Dry ice
Documentation
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