CELL LINES
Contributor Information
- Name Allen Liu
- Institute University of Michigan
Tool Details
- Tool name: Retinal pigmented epithelial cell line overexpressing HA-FLAG-CXCR4
- Tool type: Cell Lines
- Parental cell line: Retinal Pigmented Epithelial cell line
- Organism: Human
- Tissue: Eye
- Description: Retinal pigment epithelial cells (RPE) stably expressed human CXCR4 when transduced with N-terminal FLAG or N-terminal FLAG and C-terminal MYC tagged CXCR4. RPE cells were used because this cell line has very low CXCR4 expression. Results can be attributed to the overexpression of CXCR4 and not be confounded by endogenous CXCR4. As an experimental model the cells showed overexpressed CXCR4 recapitulate endogenous CXCR4 signalling and internalization dynamics.
- Research area: Cell signaling and signal transduction
- Production details: Stable RPE cell lines expressing HA-FLAG-CXCR4 or HA-FLAG-CXCR4-MYC receptors were generated using lentiviral transduction produced from the pLVX vector
- For Research Use Only
Target Details
- Target: CXCR4
Application Details
Handling
- Format: Frozen
- Shipping conditions: Dry ice
Documentation
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