CELL LINES
Contributor Information
- Name Mark Donowitz
- Institute Johns Hopkins University
Tool Details
- Tool name: NHE-1 expressing PS120 cell line
- Tool type: Cell Lines
- Parental cell line: CCL39
- Disease: Cardiac ischemia
- Description: An isoform of the Na+/H+ exchanger, NHE-1, has been cloned, sequenced and stably expressed in PS120 fibroblast cells. PS120 being a Na+/H+ exchanger deficient derivative of the CCL39 cell line, poses no background in experimental studies. The NHE cDNA was cloned and sequenced from a rabbit ileal villus cell cDNA library. The cells were then stably transfected using double selection with G418 and acid selection. The Na+/H+ exchange activity of these cells were proven using fluorescence measurement using a pH sensitive dye. These cells are ideal for the Na+/H+ exchanger functional studies as well as drug effect studies with null background. Additional advantages include:ÄË?Â? NHE-1 aids in the development of treatment for cardiac ischemia
- Research area: Drug development; Cell signaling and signal transduction
- For Research Use Only
Target Details
- Target: Na+/H+ exchanger
Application Details
Handling
- Format: Frozen
- Shipping conditions: Dry ice
Documentation
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