CELL LINES
Contributor Information
- Name Pranab K Das
Tool Details
- Tool name: Halo nevus Melanocyte Cell Line
- Alternate names: Sutton nevi, Leukoderma acquisitum centrifugum, Perinevoid vitiligo
- Tool type: Cell Lines
- Parental cell line: Halo nevus
- Organism: Human
- Disease: Halo Nevus
- Model: Primary line
- Conditional: No
- Description: Halo nevus is a mole that is surrounded by a depigmented ring or 'halo'. The formation of a halo surrounding a nevi us believed to occur when CD8+ T lymphocytes destroy the pigment producing melanocytes cells however the cause for the attack is unknown. Halo nevi are estimated to be present in approximately 1% of the general population, and are found to be more prevalent in people with vitiligo, malignant melanoma, or Turner syndrome. Primary melanocytes isolated from a halo nevus can be used as a tool for research.
- Research area: Cell biology; Immunology
- Production details: Autologous skin was taken from halo nevus using a dermatome. Epidermal cell suspension was isolated from the skin sample and seeded in cell culture medium consisting of HAMs F10 supplemented with 10ÄÂ??g/ml 12-0-tetradecanoylphorbol 13-acetate (PMA), 0.1nM isobutyl-methyl-1-xanthine (IBMX), 1% Ultroser G, 2mM glutamine, 100 IU/ml penicillin and 100ÄÂ??g/ml streptomycin. Overgrowth of fibroblasts and keratinocytes was prevented by addition of geneticine 1/100 (G418).
- For Research Use Only
Target Details
Application Details
Handling
- Format: Frozen
- Growth medium: HAMs F10 supplemented with 10 ??g/ml 12-0-tetradecanoylphorbol 13-acetate (PMA), 0.1nM isobutyl-methyl-1-xanthine (IBMX), 1% Ultroser G, 2mM glutamine, 100 IU/ml penicillin and 100??g/ml streptomycin. Overgrowth of fibroblasts and keratinocytes was prevented by addition of geneticine 1/100 (G418)
- Shipping conditions: Dry ice
Documentation
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