CELL LINES
Contributor Information
- Name Pranab K Das
Tool Details
- Tool name: Piebaldism Melanocyte Cell Line
- Tool type: Cell Lines
- Parental cell line: Lesional skin of piebaldism patient
- Organism: Human
- Disease: Piebaldism
- Model: Primary line
- Conditional: No
- Description: PiebaldismÄÂ? is a rareÄÂ? autosomal dominantÄÂ? disorder ofÄÂ? melanocyteÄÂ? development. Common characteristics include a congenital whiteÄÂ? forelock, scattered normal pigmented and hypo pigmentedÄÂ? maculesÄÂ? and a triangular shaped depigmented patch on the forehead.In some cases, piebaldism occurs together with severe developmental problems, as in Waardenburg syndrome and Hirschsprung's disease. Primary melanocytes isolated from a patient with piebaldism can be used as a tool for research
- Research area: Cell biology; Immunology
- Production details: Autologous skin was taken from patients with piebaldism using a dermatome. Epidermal cell suspension was isolated from the skin sample and seeded in cell culture medium consisting of HAMs F10 supplemented with 10ÄÂ??g/ml 12-0-tetradecanoylphorbol 13-acetate (PMA), 0.1nM isobutyl-methyl-1-xanthine (IBMX), 1% Ultroser G, 2mM glutamine, 100 IU/ml penicillin and 100ÄÂ??g/ml streptomycin. Overgrowth of fibroblasts and keratinocytes was prevented by addition of geneticine 1/100 (G418).
- For Research Use Only
Target Details
Application Details
Handling
- Format: Frozen
- Growth medium: HAMs F10 supplemented with 10 ??g/ml 12-0-tetradecanoylphorbol 13-acetate (PMA), 0.1nM isobutyl-methyl-1-xanthine (IBMX), 1% Ultroser G, 2mM glutamine, 100 IU/ml penicillin and 100??g/ml streptomycin. Overgrowth of fibroblasts and keratinocytes was prevented by addition of geneticine 1/100 (G418)
- Shipping conditions: Dry ice
Documentation
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