CELL LINES
Contributor Information
- Name Irene Leigh
- Institute Queen Mary University of London
Tool Details
- Tool name: T11 SCC Cell Line
- Alternate names: SCC, Squamous cell carcinoma, T11
- Tool type: Cell Lines
- Organism: Human
- Tissue: Skin
- Cancer type: Skin cancer; Skin cancer
- Disease: Cancer
- Model: Tumour line
- CRISPR: No
- Conditional: No
- Description: The SCC series represent primary, recurrent and metastatic carcinoma. Availability of such a series from the same individual will facilitate genetic analysis of the malignant process.
- Research area: Cancer
- Production details: Cutaneous SCC cell line from 48 year old male. Poorly defined primary tumour from the back with germline DNA from lymphocytes. Patient had a renal transplant and was treated with Cyclosporine, Prednisolone and Azathioprine. Patient had stopped Azathioprine treatment at the time of lesional biopsy.
- Additional notes: IC1 and IC1MET SCC Cell Lines cells are the most successful in xenografts and surface xenotransplantation (unpublished)
- For Research Use Only
Target Details
- Target: Squamous Cell Carcinoma
Application Details
Handling
- Format: Frozen
- Growth medium: Keratinocyte medium: 3:1 V/V mixture of DMEM and Ham’s F12 supplemented with 10% FBS and a cocktail of mitogens: 0.5 ug/ml hydrocortisone; 1x10-10M cholera toxin; 1.8 x 10-4 M adenine; 2 x 10–11 M liothyronine, 13 ng/ml; 5 μg/mL insulin; 10 ng/mL epidermal growth factor (EGF). FBS (10%) is routinely batch tested for enabling optimal clonal growth of cells. Culture SCC cell lines in the presence of mitotically inactivated 3T3-J2 feeder layers, which enhance proliferative capacity and reduces selective pressure on the cancer cells.
- Storage conditions: Liquid Nitrogen
- Shipping conditions: Dry ice
- Characterisation tests: Genetic analysis [PMID: 30202019]
- Mycoplasma free: Yes
- Biosafety level: 1
Documentation
References
- • Inman et al. 2018. Nat Commun. 9(1):3667. PMID: 30202019.
- • The genomic landscape of cutaneous SCC reveals drivers and a novel azathioprine associated mutational signature.
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