CELL LINES
Contributor Information
- Name Irene Leigh
- Institute Queen Mary University of London
Tool Details
- Tool name: PM1 Cell Line
- Alternate names: Keratinocyte, epithelium
- Tool type: Cell Lines
- Tool sub-type: Primary
- Organism: Human
- Tissue: Skin
- Model: Primary line
- CRISPR: No
- Conditional: No
- Description: A unique series of epidermal cell lines representing different stages of malignant transformation were spontaneously derived from a single adult immunosuppressed individual. Four keratinocyte lines (PM1-4) established from forehead skin are here compared with 4 squamous cell carcinoma (SCC) lines (MET1-4) derived respectively from a primary cutaneous tumour, two local recurrences and a distant metastasis of invasive SCC. Despite altered growth properties, the PM lines retained many features of normal keratinocytes including keratin phenotype, differentiation capacity and non-tumorigenicity in athymic mice. In contrast, from early passage, the MET lines displayed markedly reduced growth requirements, abnormal differentiation, aberrant K18 expression and tumorigenicity in athymic mice. The abnormal keratin profile of individual MET lines closely reflected the keratin phenotype of the tumour of origin. Although unusual HPV types were identified in the original tissue, there was no evidence of persistent virus within any cell line and it appears that HPV is not critical for maintenance of the immortal phenotype. The PM lines were distinctly different from invasive SCC lines and are likely to be useful for studies of mutations important early in neoplastic progression. The SCC series represent primary, recurrent and metastatic carcinoma. Availability of such a series from the same individual will facilitate genetic analysis of the malignant process.
- Research area: Cancer; Cell biology; Developmental biology
- Production details: PM1 was cloned from a culture of dysplastic forehead skin that showed extended life span in vitro.
- Additional notes: IC1 and IC1MET SCC Cell Lines cells are the most successful in xenografts and surface xenotransplantation (unpublished)
- For Research Use Only
Target Details
- Target: Keratinocyte line
Application Details
Handling
- Format: Frozen
- Growth medium: Keratinocyte medium: 3:1 V/V mixture of DMEM and Ham’s F12 supplemented with 10% FBS and a cocktail of mitogens: 0.5 ug/ml hydrocortisone; 1x10-10M cholera toxin; 1.8 x 10-4 M adenine; 2 x 10–11 M liothyronine, 13 ng/ml; 5 μg/mL insulin; 10 ng/mL epidermal growth factor (EGF). FBS (10%) is routinely batch tested for enabling optimal clonal growth of cells. Culture SCC cell lines in the presence of mitotically inactivated 3T3-J2 feeder layers, which enhance proliferative capacity and reduces selective pressure on the cancer cells.
- Storage conditions: Liquid Nitrogen
- Shipping conditions: Dry ice
- Characterisation tests: Genetic analysis [PMID: 30202019]
- Mycoplasma free: Yes
- Biosafety level: 1
Documentation
Related Tools
References
- • Spontaneous keratinocyte cell lines representing early and advanced stages of malignant transformation of the epidermis. Proby et al. 2000. Exp Dermatol. 9(2):104-17. PMID: 10772384.
- • Spontaneous keratinocyte cell lines representing early and advanced stages of malignant transformation of the epidermis.
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