CELL LINES
Contributor Information
- Name Irene Leigh
- Institute Queen Mary University of London
Tool Details
- Tool name: MET2 SCC Cell Line
- Alternate names: SCC, Keratinocyte
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Clone: MET2
The difference between clones: keratinocyte lines MET1, MET2, MET3 and MET4 were derived from the same individual, but from varying stages in progression of an SCC: invasive, recurrent and, subsequently metastatic.
The clones were derived, respectively, from an SCC excised from the back of the left hand (MET1), from 2 recurrent SCC arising at the same site in rapid succession (MET2 and 3) and from metastatic SCC within left axillary lymph nodes biopsied following the third excision (MET4). - Tool type: Cell Lines
- Organism: Human
- Donor: Single immunosuppressed patient, from a primary invasive SCC on the back of the hand that subsequently recurred locally twice
- Tissue: Skin
- Cancer type: Skin cancer; Skin cancer
- Disease: Cancer
- Model: Tumour line
- CRISPR: No
- Conditional: No
- Description: Keratinocyte cell line representing a recurring acantholytic carcinoma that originating in the same site as the primary invasive and ultimately metastatic SCC tumour. Background and Research Application MET2 is derived from a recurrent epidermal tumour from the back of a left hand, which in time progressed through to an invasive and metastatic lesion. Histology of the original tumour showed an acantholytic squamous cell carcinoma. MET2 has been used to understand aggressive recurring SCCs which ultimately metastasise and is an important cell line for this specific stage in SCC cancer progression. MET2 is the second cell line in a unique series of epidermal cell lines representing different stages of malignant transformation were derived from a single adult immunosuppressed individual. Four keratinocyte lines (PM1-4) established from forehead skin are here compared with 4 squamous cell carcinoma (SCC) lines (MET1-4) derived respectively from a primary cutaneous tumour, two local recurrences and a distant metastasis of invasive SCC. Despite altered growth properties, the PM lines retained many features of normal keratinocytes including keratin phenotype, differentiation capacity and non-tumorigenicity in athymic mice. In contrast, from early passage, the MET lines displayed markedly reduced growth requirements, abnormal differentiation, aberrant K18 expression and tumorigenicity in athymic mice. The abnormal keratin profile of individual MET lines closely reflected the keratin phenotype of the tumour of origin. Although unusual HPV types were identified in the original tissue, there was no evidence of the persistent virus within any cell line and it appears that HPV is not critical for maintenance of the immortal phenotype. The PM lines were distinctly different from invasive SCC lines and are likely to be useful in studies focusing on mutations that are important in early neoplastic progression. The SCC series represent primary, recurrent and metastatic carcinoma. Availability of such a series from the same individual will facilitate genetic analysis of the malignant process.
- Research area: Cancer
- Production details: MET2 was derived from a recurrent SCC excised from the back of the left hand
- Additional notes: DiffereIC1 and IC1MET SCC Cell Lines cells are the most successful in xenografts and surface xenotransplantation (unpublished)nce Between ClonesAll derived from the same individual, but from varying stages in progression of an SCC: invasive, recurrent and, subsequently metastatic. Keratinocyte lines MET1, MET2, MET3 and MET4 were derived, respectively, from an SCC excised from the back of the left hand (MET1), from 2 recurrent SCC arising at the same site in rapid succession (MET2 and 3)and from metastatic SCC within left axillary lymph nodes biopsied following the third excision (MET4).
- For Research Use Only
Target Details
- Target: Squamous Cell Carcinoma line
Application Details
- Application notes: Points of Interest MET cell lines were derived from a single immunosuppressed patient, from a primary invasive SCC on the back of the hand that subsequently recurred locally twice. Difference Between Clones All derived from the same individual, but from varying stages in progression of an SCC: invasive, recurrent and, subsequently metastatic. Keratinocyte lines MET1, MET2, MET3 and MET4 were derived, respectively, from an SCC excised from the back of the left hand (MET1), from 2 recurrent SCC arising at the same site in rapid succession (MET2 and 3)and from metastatic SCC within left axillary lymph nodes biopsied following the third excision (MET4). Concentration Vial has between 1-5 million cells as standard, however this may vary.
Handling
- Format: Frozen
- Growth medium: Keratinocyte medium: 3:1 V/V mixture of DMEM and Ham’s F12 supplemented with 10% FBS and a cocktail of mitogens: 0.5 ug/ml hydrocortisone; 1x10-10M cholera toxin; 1.8 x 10-4 M adenine; 2 x 10–11 M liothyronine, 13 ng/ml; 5 μg/mL insulin; 10 ng/mL epidermal growth factor (EGF). FBS (10%) is routinely batch tested for enabling optimal clonal growth of cells. Culture SCC cell lines in the presence of mitotically inactivated 3T3-J2 feeder layers, which enhance proliferative capacity and reduces selective pressure on the cancer cells.
- Storage conditions: Liquid Nitrogen
- Shipping conditions: Dry ice
- Characterisation tests: Genetic analysis [PMID: 30202019]
- Mycoplasma free: Yes
- Biosafety level: 1
Documentation
Related Tools
References
- • Inman et al. 2018. Nat Commun. 9(1):3667. PMID: 30202019.
- • The genomic landscape of cutaneous SCC reveals drivers and a novel azathioprine associated mutational signature.
- • Abikhair Burgo et al. 2018. JCI Insight. 3(17):. PMID: 30185657.
- • Abikhair Burgo et al. 2018. JCI Insight. 3(17):. PMID: 30185657.
- • Mekhdjian et al. 2017. Mol Biol Cell. 28(11):1467-1488. PMID: 28381423.
- • Proby et al. 2000. Exp Dermatol. 9(2):104-17. PMID: 10772384.
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