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Contributor Information

  • Name Sandra Van Schaeybroeck
  • Institute Queen's University Belfast

Tool Details

  • Tool name: HCT 116-I6 Invasive Cell Line
  • Tool type: Cell Lines
  • Tool sub-type: Continuous
  • Parental cell line: HCT 116
  • Organism: Human
  • Tissue: Colon
  • Cancer type: Digestive / Gastrointestinal cancer
  • Disease: Cancer
  • Growth properties: Invasion, migration
  • Model: Cancer Model
  • Conditional: No
  • Research area: Cancer; Cell biology; Drug development
  • Production details: HCT 116 cells were incubated in a Matrigel Invasion Chambers (MIC) for 72-96h. The cells that invaded through to the bottom chamber were collected and designated as â?‚€?‚˜Invasive 1â?‚€?‚™ (I1). These cells were propagated and repeatedly passed through the MIC until highly invasive HCT116-I6 cells were selected.
  • Cellosaurus ID: CVCL_HG06

  • For Research Use Only

Target Details

Application Details

Handling

  • Format: Frozen
  • Growth medium: McCoy's 5a Medium (GIBCO # 16600) + 10% FBS + 100 units/ml penicillin+ 100 ?g/ml streptomycin
  • Shipping conditions: Dry ice

Documentation

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References

  • •  Dunne et al. 2016. Clin Cancer Res. 22(1):230-42. PMID: 26283684.
  • •  EphA2 Expression Is a Key Driver of Migration and Invasion and a Poor Prognostic Marker in Colorectal Cancer.
  • •  Dunne et al. 2014. Clin Cancer Res. 20(1):164-75. PMID: 24170546.
  • •  AXL is a key regulator of inherent and chemotherapy-induced invasion and predicts a poor clinical outcome in early-stage colon cancer.