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Contributor Information

  • Name Anne Lykkesfeldt
  • Institute Danish Cancer Society, Denmark
  • Primary citation Jensen et al. 2003. J Steroid Biochem Mol Biol. 84(4):469-478. PMID: 12732292.

Tool Details

  • Tool name: MCF7/S9 Cell Line
  • Alternate names: MCF-7/S9; S9
  • Tool type: Cell Lines
  • Tool sub-type: Continuous
  • Parental cell line: MCF7
  • Organism: Human
  • Tissue: Breast
  • Gender: Female
  • Cancer type: Breast cancer
  • Disease: Cancer
  • Growth properties: Grow in serum free medium. Estrogen and progesterone receptor positive. Estrogen and antiestrogen responsive.
  • Conditional: No
  • Application: Evaluation of antioestrogens inhibitory effects
  • Description: MCF-7/S9 cell line is an oestrogen-responsive (not oestrogen-dependent) breast cancel model. This model is derived from MCF-7, a human breast carcinoma line with epithelial like morphology.

    The MCF-7/S9 cell line was established to grow in serum free medium.

    Previous findings of the MCF-7/S9 cell line include antioestrogens, specifically tamoxifen, fulvestrant and acolbifene exert a significant direct growth-inhibitory effect.

    This model enables investigation of the effect of oestrogens and antioestrogens on tumour cell growth under conditions without influence of oestrogenic compounds supplied via the serum content in the growth medium.
  • Research area: Cancer; Drug development
  • Production details: MCF7/S9 has been established by gradually adapting MCF7 cells to grow at a low serum concentration before transfer to serum-free medium. Since passage no. 387, the cell line has been propagated in phenol red-free DMEM/F12 (1:1) without addition of serum or any growth factor (standard medium for MCF7/S9), in T-flasks coated with collagen. The MCF7/S9 was subcultured once a week with a split ratio of approximately 1:10 and growth medium was changed every second or third day.
  • Cellosaurus ID: CVCL_AS09

  • For Research Use Only

Target Details

Application Details

  • Application: Evaluation of antioestrogens inhibitory effects

Handling

  • Format: Frozen
  • Growth medium: Phenol-red-free DMEM/F12 medium supplemented with 2.0 mM Glutamax, 10 ug/ml Transferrin, 0.1 mg/ml bovine serum albumin, culture flasks were coated with collagen IV.
  • Shipping conditions: Dry ice

Documentation

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References

  • •  Jensen et al. 2003. J Steroid Biochem Mol Biol. 84(4):469-478. PMID: 12732292.
  • •  Briand et al. 1986. Anticancer Res. 6(1):85-90. PMID: 3513694.