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Contributor Information

  • Name Joyce Taylor-Papadimitriou ; Sidney Chang
  • Institute Cancer Research UK, Lincoln's Inn Fields Institute
  • Primary citation Yamashita et al. 2009. Bioorg Med Chem. 17(17):6286-6291. PMID: 19674905.

Tool Details

  • Tool name: SVCT-MI2 Cell Line
  • Tool type: Cell Lines
  • Organism: Human
  • Tissue: Breast
  • Gender: Female
  • Cancer type: Breast cancer
  • Disease: Cancer
  • Growth properties: Adherent
  • Conditional: No
  • Application: Molecular analysis of SV40 in breast cancer
  • Description: SVCT-MI2 has been established from human breast epithelial cells being transfected with simian virus 40 (SV40) - a known oncogenic DNA virus in addition to a plasmid containing the neomycin gene and the human oncogene EJ Harvey-ras.

    This transformed epithelial cell line shows tumourigenicity in nude mice (post-selection of G418-resistant colonies).

    SVCT-MI2 enables molecular analysis of SV40 in human breast cancers.
  • Research area: Cancer; Cell biology; Drug development; Genetics
  • Cellosaurus ID: CVCL_2719

  • For Research Use Only

Target Details

  • Target: Human oncogene EJ Harvey-ras

Application Details

  • Application: Molecular analysis of SV40 in breast cancer

Handling

  • Format: Frozen
  • Growth medium: RPMI 1640 or DMEM + 2mM Glutamine + 10ug / ml insulin + 5ug / ml hydrocortisone + 10% FBS. Subculture routine: Split sub-confluent cultures (70-80%) 1:4 to 1:10 using 0.25% trypsin or trypsin/EDTA. Note: cells may pile up when confluent.
  • Temperature: 37° C
  • Atmosphere: 5% CO2
  • Shipping conditions: Dry ice

Documentation

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References

  • •  Yamashita et al. 2009. Bioorg Med Chem. 17(17):6286-6291. PMID: 19674905.