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Contributor Information

  • Name Anne Lykkesfeldt
  • Institute Danish Cancer Society
  • Primary citation Kirkegaard et al. 2014. Cancer Lett. 344(1):90-100. PMID: 24513268.

Tool Details

  • Tool name: T47D/TR-1 Cell Line
  • Tool type: Cell Lines
  • Tool sub-type: Continuous
  • Parental cell line: T47D/S2
  • Organism: Human
  • Tissue: Breast
  • Cancer type: Breast cancer
  • Disease: Cancer
  • Model: Tumour line
  • Conditional: Yes
  • Application: Determining molecular mechanisms around tamoxifen resistance
  • Description: The T47D/TR-1 cell line is a breast cancer cell line resistant to fulvestrant (Faslodex). This cell line has been established from T47D/S2 cells by long term treatment with 1 nM tamoxifen. T47D/TR-1 is adherent and the morphology is polygonal epithelial.

    T47D/TR-1 cells are oestrogen receptor (alpha) positive, they also express progesterone receptor. T47D/TR-1 are growth inhibited by fulvestrant.

    Antioestrogen resistance is a major problem in breast cancer treatment. Therefore, the search for new therapeutic targets and biomarkers for antiestrogen resistance is crucial. This cell line allows the study of the mechanisms involved in tamoxifen resistant breast cancer cell growth.
  • Research area: Cancer; Drug development
  • Production details: The T47D/TR-1 cell line has been established by long term treatment of T47D/S2 cells with 1 uM tamoxifen. Clonal selection was performed in medium without tamoxifen. After growth in presence of 1 uM tamoxifen for 10 months, the growth rate was similar to the parental cells.
  • Cellosaurus ID: CVCL_1D36

  • For Research Use Only

Target Details

  • Target: Oestrogen receptor

Application Details

  • Application: Determining molecular mechanisms around tamoxifen resistance
  • Application notes: T47D/TR-1 cells are ER alpha positive and express progesterone receptor, although at reduced level compared to parental T7D/S2 cells. T47D/TR-1 are growth inhibited by fulvestrant.

Handling

  • Format: Frozen
  • Passage number: Passage 164 (AL3569, AL3570)
  • Growth medium: Phenol red free RPMI 1640 + 2% FCS + glutamax + 8ug Insulin/ml + 1 uM tamoxifen
  • Temperature: 37° C
  • Atmosphere: 5% CO2
  • Storage conditions: Liquid Nitrogen
  • Shipping conditions: Dry ice
  • Mycoplasma free: Yes
  • Biosafety level: 1

Documentation

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References

  • •  Larsen et al. 2015. PLoS One. 10(2):e0118346. PMID: 25706943.
  • •  Larsen et al. 2015. BMC Cancer. 15(1):1-15. PMID: 25885472.
  • •  Thrane et al. 2014. Oncogene. 34(32):4199-4210. PMID: 25362855.
  • •  Kirkegaard et al. 2014. Cancer Lett. 344(1):90-100. PMID: 24513268.