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Contributor Information

  • Name Joyce Taylor-Papadimitriou ; Joy Burchell
  • Institute Cancer Research UK, Lincoln's Inn Fields Institute

Tool Details

  • Tool name: 1-7 CE1 Cell Line
  • Tool type: Cell Lines
  • Tool sub-type: Continuous
  • Parental cell line: MSTV1-7 HER2
  • Organism: Human
  • Tissue: Breast
  • Gender: Female
  • Cancer type: Breast cancer
  • Disease: Cancer
  • Model: Transgenic
  • Application: Investigation of ERBB2 proto-oncogene in breast cancer
  • Description: 1-7 CE1 is a clonal derivative of the human mammary luminal epithelial cell line MTSV1-7. MTSV1-7 is transfected with erb-b2 receptor tyrosine kinase 2 (ERBB2) expression vector, enabling the over-expression of ERBB2 proto-oncogene. At confluence, the morphology is cuboidal.

    The ERBB2 (or Her2) oncogene is amplified and/or overexpressed in a significant proportion of breast cancers. The over-expression of the ERBB2 receptor inhibits morphogenesis in vitro, and induces reduced expression of surface adhesion molecules such as E-cadherin, suggesting a role for ERBB2 over-expression in tumour progression and metastasis.

    The 1-7 CE1 cell line is an ideal in vitro tool for investigation of the role of the ERBB2 proto-oncogene in breast cancer morphogenesis and adhesion.
  • Research area: Cell biology; Cancer; Cell signaling and signal transduction; Drug development
  • Production details: MTSV1-7 transfected with SV40 plasmid DNA; MTSV1-7 ce1 line transfected with plasmid constructs for pSV2-erbB2 and pSV2neo.
  • Cellosaurus ID: CVCL_3033

  • For Research Use Only

Target Details

  • Target: ERBB2

Application Details

  • Application: Investigation of ERBB2 proto-oncogene in breast cancer

Handling

  • Format: Frozen
  • Growth medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + Insulin (bovine) at 10ug/ml + Hydrocortisone at 5ug/ml.
  • Temperature: 37° C
  • Atmosphere: 5% CO2
  • Shipping conditions: Dry ice

Documentation

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References

  • •  D'souza et al. 1994. Proc Natl Acad Sci U S A. 91(15):7202-7206. PMID: 7913748.
  • •  D'Souza et al. 1993. Oncogene. 8(7):1797-1806. PMID: 8099725.
  • •  Bartek et al. 1991. Proc Natl Acad Sci U S A. 88(9):3520-3524. PMID: 1708884.