ANTIBODIES
Contributor Information
- Name Irwin Bernstein
- Institute Fred Hutchinson Cancer Research Center
- Primary citation Andrews etal.1983. Blood. 62(1):124-32. PMID: 6190518
Tool Details
- Tool name: Anti-CFU-GM
- Clone: T5A7
- Tool type: Antibody
- Tool sub-type: Primary Antibody
- Class: Monoclonal
- Reactivity: Human
- Host: Mouse
- Application: Indirect Immunofluorescent Staining, Fluorescence-Activated Cell Sorting
- Strain: Application Notes: They are potentially useful for depleting or enriching myeloid cell populations, including committed hematopoietic precursor cells. They provide probes to examine cell surface biochemical changes and eventually the responsible genetic alterations that occur during normal as well as malignant myeloid differentiation. They relate the cell surface phenotype of malignant myeloid cells to stages of normal myelocytic differentiation based on the expression of maturation-linked antigens.
- Description: This antibody will react with both granulocytic and monocytic cells. It is potentially useful for depleting or enriching myeloid cell populations, including committed hematopoietic precursor cells.
- Isotype: IgM
- Research area: Stem cell biology
- For Research Use Only
Target Details
- Target: CFU-GM (granulocyte-macrophage colony-forming units)
- Target background: Myeloid-associated determinants on committed myeloid stem cells and their progeny.
Application Details
- Application: Indirect Immunofluorescent Staining, Fluorescence-Activated Cell Sorting
- Application notes: A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB)
Handling
- Storage conditions: . For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Documentation
Related Tools
References
- • Andrews etal.1983. Blood. 62(1):124-32.PMID: 6190518
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