ANTIBODIES
Contributor Information
- Name Irwin Bernstein
- Institute Fred Hutchinson Cancer Research Center
- Primary citation Hamann et al.2002. Bioconjugate Chemistry. 13(1]:47-58. PMID: 11792178
Tool Details
- Tool name: Anti-CD33
- Clone: P67.6
- Tool type: Antibody
- Tool sub-type: Primary Antibody
- Class: Monoclonal
- Reactivity: Human
- Host: Mouse
- Cancer type: Blood cancer
- Molecular weight of the target: 39.82 kDa
- Application: Flow Cytometry, Immunocytochemistry, Inhibition assays.
- Description: Anti-CD33, clone P676 is a mouse monoclonal antibody that detects CD33
- Immunogen: FMY9S5 cells expressing CD33
- Isotype: IgG1,?
- Research area: Cell biology
- For Research Use Only
Target Details
- Target: CD33
- Target molecular weight: 39.82 kDa
- Target background: CD33 is expressed by acute myeloid leukemia (AML) cells in >80% of patients but not by normal hematopoietic stem cells, suggesting that elimination of CD33+ cells may be therapeutically beneficial.
Application Details
- Application: Flow Cytometry, Immunocytochemistry, Inhibition assays.
- Application notes: A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB)
Handling
Documentation
Related Tools
References
- • Hamann et al.2002. Bioconjugate Chemistry. 13(1]:47-58. PMID: 11792178
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