ANTIBODIES
Contributor Information
- Name Elizabeth A. Wayner
- Institute Fred Hutchinson Cancer Research Center
- Primary citation Garcia-Pardo et al.1990. Journal of Immunology.144(9):3361-6. PMID: 2139453
Tool Details
- Tool name: Anti-integrin b2
- Clone: P4H9
- Tool type: Antibody
- Tool sub-type: Primary Antibody
- Class: Monoclonal
- Reactivity: Human
- Host: Mouse
- Molecular weight of the target: Predicted: 85 kDa; Apparent: 93 kDa
- Application: FACS, Function Blocking, Immunohistochemistry
- Description: CD18 is a general marker of leukocytes.
- Immunogen: Human lymphokine activated T-cells (LAK)
- Isotype: IgG3
- Research area: Cell biology
- For Research Use Only
Target Details
- Target: CD18 (integrin ?2; ? subunit of CD11a, b, c)
- Target molecular weight: Predicted: 85 kDa; Apparent: 93 kDa
- Target background: CD18 (integrin ?2; ? subunit of CD11a, b, c)
Application Details
- Application: FACS, Function Blocking, Immunohistochemistry
- Application notes: A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
Handling
- Storage conditions: For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Documentation
Related Tools
References
- • Garcia-Pardo et al.1990. Journal of Immunology.144(9):3361-6.PMID: 2139453
- • Languino et al.1989. J Cell Biol.109(5):2455-62.PMID: 2530239
- • Dittel et al.1993.Blood.81(9):2272-82.PMID: 7683214
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