ANTIBODIES
Contributor Information
- Name Beverly TorokStorb
- Institute Fred Hutchinson Cancer Research Center
- Primary citation Simmons et al. 1991.Blood.78(1)55-62. PMID: 2070060
Tool Details
- Tool name: Anti-STRO-1
- Clone: STRO1
- Tool type: Antibody
- Tool sub-type: Primary Antibody
- Class: Monoclonal
- Reactivity: Human, Mouse, Primate
- Host: Mouse
- Application: ELISA, FACS, FFPE, Function Blocking, Immunofluorescence, Immunohistochemistry
- Description: Murine IgM monoclonal antibody STRO-1 identifies a cell surface antigen expressed by stromal elements in human bone marrow (BM). STRO-1 binds to approximately 10% of BM mononuclear cells, greater than 95% of which are nucleated erythroid precursors, but does not react with committed progenitor cells (colony-forming unit granulocyte-macrophage [CFU-GM], erythroid bursts [BFU-E], and mixed colonies [CFU-Mix]). STRO-I binds to BM stromal elements with the capacity to transfer the hematopoieticmicroenvironment in vitro.
- Immunogen: CD34 positive (CD34+) Bone Marrow (BM) cells
- Isotype: IgM
- Research area: Immunology; Stem cell biology
- For Research Use Only
Target Details
- Target: STRO-1 , Mesenchymal stem cell marker
- Target background: STRO-1 binds to human bone marrow(BM) fibroblast-like cells and to various nonhematopoietic cellular components of the adherent cell layer in long-term BM cultures (LTBMC). STRO-1 antibody can also be used to isolate stromal precursors in freshly aspirated BM cell suspensions and has the capacity to transfer the hematopoietic microenvironment in vitro.
Application Details
- Application: ELISA, FACS, FFPE, Function Blocking, Immunofluorescence, Immunohistochemistry
- Application notes: A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
Handling
- Storage conditions: For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Documentation
Related Tools
References
- • Simmons et al. 1991.Blood.78(1)55-62. PMID: 2070060
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