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Contributor Information

  • Name Richard Nash
  • Institute Fred Hutchinson Cancer Research Center
  • Primary citation McSweeney et al. 1998.Blood. 91(6):1977-86. PMID: 9490680

Tool Details

  • Tool name: Anti-CD34 [1H6]
  • Clone: 1H6
  • Tool type: Antibody
  • Tool sub-type: Primary Antibody
  • Class: Monoclonal
  • Reactivity: Human
  • Host: Canine
  • Molecular weight of the target: 90kDa
  • Application: Western blotting, flow cytometry, CFU-GM assay, LTC-IC assay, ELISA
  • Description: Good animal models in which to refin studies of CD34 cell fractions are limited. However, this MoAb detected 4% to 17% CD34 cells in murine Bone Marrow(BM) cells, somewhat higher than the 1% to 5% CD34 cells detected in human BM.
  • Immunogen: A recombinant CD34 murine Ig fusion molecule (CD34-Ig)
  • Isotype: IgG-1
  • Research area: Stem cell biology

  • For Research Use Only

Target Details

  • Target: CD34
  • Target molecular weight: 90kDa
  • Target background: CD34 has previously been define as a marker of hematopoietic progenitors and is expressed on canine bone marrow (BM) progenitor cells in a manner analogous to that found in humans.

Application Details

  • Application: Western blotting, flow cytometry, CFU-GM assay, LTC-IC assay, ELISA
  • Application notes: A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).

Handling

  • Storage conditions: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided.

Documentation

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References

  • •  McSweeney et al. 1998.Blood. 91(6):1977-86.PMID:PMID: 9490680
  • •  Wayner et al. 1987.J Cell Biol.105 (1987): 1873.https://doi.org/10.1083/jcb.105.4.1873