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Contributor Information

  • Name Lynda Partridge
  • Institute University of Sheffield

Tool Details

  • Tool name: Anti-F-actin [NH3]
  • Alternate names: Actin Antibody, Anti-Actin Antibody, F-Actin, f actin antibody, Filamentous actin antibody
  • Clone: NH3
  • Tool type: Antibodies
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Chicken ; Human ; Mouse ; Rabbit
  • Host: Mouse
  • Application: ELISA ; FACS
  • Description: Monoclonal antibody used to study the localisation and interactions of F-actin in all cell types. Background and Research Application Antibody NH3 has been shown to recognize actin. The epitope recognized is preserved across species (mouse, rabbit and human) and for different isoforms. NH3 recognises human filamentous actin (F-actin), but has been reported to recognise globular actin (G-actin). NH3 therefore represents a useful probe for the localization of actin and the study of its interaction with other molecules. NH3 strongly stains the cytoplasm of macrophages and causes granular, localised nuclear staining of all cell types. NH3 also reacts with the cell surface of cell lines that originate from B-cells.
  • Immunogen: Human monocytes and the human monocyte-like cell line, U937.
  • Immunogen UniProt ID: P60709
  • Isotype: IgM kappa
  • Research area: Cell biology; Developmental biology

  • For Research Use Only

Target Details

  • Target: Filamentous Actin
  • Target background: Monoclonal antibody used to study the localisation and interactions of F-actin in all cell types Background and Research Application Antibody NH3 has been shown to recognize actin. The epitope recognized is preserved across species (mouse, rabbit and human) and for different isoforms. NH3 recognises human filamentous actin (F-actin), but has been reported to recognise globular actin (G-actin). NH3 therefore represents a useful probe for the localization of actin and the study of its interaction with other molecules. NH3 strongly stains the cytoplasm of macrophages and causes granular, localised nuclear staining of all cell types. NH3 also reacts with the cell surface of cell lines that originate from B-cells.

Application Details

  • Application: ELISA ; FACS

Handling

  • Format: Liquid
  • Concentration: 1mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Initial characterization of an anti-actin monoclonal antibody (NH3) DOI: 10.1042/bst0160163