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Contributor Information

  • Name Birgit Lane
  • Institute University of Dundee

Tool Details

  • Tool name: Anti-Ribophorin-1 [CEL5C]
  • Alternate names: OST1; RBPH1
  • Clone: CEL5C
  • Tool type: Antibodies
  • Tool sub-type: Primary Antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Xenopus laevis
  • Host: Mouse
  • Molecular weight of the target: 65 kDa
  • Application: IP ; WB
  • Description: Ribophorin I is an essential subunit of oligosaccharyltransferase (OST), which is also known as dolichyl-diphosphooligosaccharide--protein glycosyltransferase. OST catalyses the transfer of an oligosaccharide from dolichol pyrophosphate to selected asparagine residues of nascent polypeptides as they are translocated into the lumen of the rough endoplasmic reticulum. Ribophorin I and OST48 are thought to be responsible for OST catalytic activity. Both yeast and mammalian proteins are glycosylated but the sites are not conserved. Glycosylation may contribute towards general solubility but is unlikely to be involved in a specific biochemical function. Most family members are predicted to have a transmembrane helix at the C terminus of this region.
  • Immunogen: Nuclei formed in vitro in Xenopus egg extract
  • Isotype: IgG1
  • Research area: Cell signaling and signal transduction

  • For Research Use Only

Target Details

  • Target: ribophorin type 1
  • Target molecular weight: 65 kDa
  • Target background: Ribophorin I is an essential subunit of oligosaccharyltransferase (OST), which is also known as dolichyl-diphosphooligosaccharide--protein glycosyltransferase. OST catalyses the transfer of an oligosaccharide from dolichol pyrophosphate to selected asparagine residues of nascent polypeptides as they are translocated into the lumen of the rough endoplasmic reticulum. Ribophorin I and OST48 are thought to be responsible for OST catalytic activity. Both yeast and mammalian proteins are glycosylated but the sites are not conserved. Glycosylation may contribute towards general solubility but is unlikely to be involved in a specific biochemical function. Most family members are predicted to have a transmembrane helix at the C terminus of this region.

Application Details

  • Application: IP ; WB

Handling

  • Format: Liquid
  • Concentration: 0.9-1.1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Addinall et al. 2001. J Biol Chem. 276(19):15939-44. PMID: 11278950.
  • •  Phosphorylation by cdc2-CyclinB1 kinase releases cytoplasmic dynein from membranes.