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Contributor Information

  • Name Lionel Crawford
  • Institute Cancer Research UK, London Research Institute: Clare Hall Laboratories

Tool Details

  • Tool name: Anti-SV40T [PAB416]
  • Clone: PAb416
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Virus
  • Host: Mouse
  • Cancer type: Broadly Applicable; Polyoma
  • Molecular weight of the target: 94 kDa
  • Application: IHC ; IF ; IP ; WB
  • Strain: Balb/c
  • Description: Monoclonal antibody directed at large T antigen of SV40, valuable for investigation into other SV40 tumour antigens and polyomaviruses. Background and Research Application Anti-SV40T which binds to the large T antigen of the Simian oncogenic polyoma virus (SV40). Both the Simian virus (SV40) large T and small T antigens are encoded by the early region of the SV40 genome. The SV40 large T antigen is an early protein that interacts with the p53 and Rb tumour suppressor proteins. Large T is capable of inducing malignant transformation within cells, predominantly due to its disruption and inactivation of the 105-retinoblastoma and p53 tumour suppressor. This causes the cell to enter the S phase of the cell cycle, promoting DNA replication. Large T interacts with DNA polymerase-alpha to directly stimulate viral DNA replication. Anti-SV40T was created in 1981 to further understand the SV40 antigens via immunochemical analysis. It was thought that detailed mapping and analysis of the SV40 tumour antigens would provide information regarding the tertiary structure and ultimately the functions of these antigens.
  • Immunogen: SV40 transformed mouse cells
  • Immunogen UniProt ID: P03070
  • Isotype: IgG2a
  • Research area: Microbiology
  • Myeloma used: P3/NS1/1-Ag4.1

  • For Research Use Only

Target Details

  • Target: N-terminal domain of SV40 large and small T-antigen
  • Target molecular weight: 94 kDa
  • Target background: Monoclonal antibody directed at large T antigen of SV40, valuable for investigation into other SV40 tumour antigens and polyomaviruses. Background and Research Application Anti-SV40T which binds to the large T antigen of the Simian oncogenic polyoma virus (SV40). Both the Simian virus (SV40) large T and small T antigens are encoded by the early region of the SV40 genome. The SV40 large T antigen is an early protein that interacts with the p53 and Rb tumour suppressor proteins. Large T is capable of inducing malignant transformation within cells, predominantly due to its disruption and inactivation of the 105-retinoblastoma and p53 tumour suppressor. This causes the cell to enter the S phase of the cell cycle, promoting DNA replication. Large T interacts with DNA polymerase-alpha to directly stimulate viral DNA replication. Anti-SV40T was created in 1981 to further understand the SV40 antigens via immunochemical analysis. It was thought that detailed mapping and analysis of the SV40 tumour antigens would provide information regarding the tertiary structure and ultimately the functions of these antigens.

Application Details

  • Application: IHC ; IF ; IP ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: Store at -20°C frozen. Avoid repeated freeze / thaw cycles
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Tanaka et al. 2018. J Vet Med Sci. 80(9):1400-1406. PMID: 30012933.
  • •  Pelletier et al. 2018. Histopathology. 73(1):162-166. PMID: 29430700.
  • •  Rigatti et al. 2016. mSphere. 1(6):. PMID: 28028546.
  • •  Toptan et al. 2016. JCI Insight. 1(2):. PMID: 27034991.
  • •  Chiaravalli et al. 2013. J Clin Pathol. 66(1):44-9. PMID: 23012397.
  • •  Sabatier et al. 2005. J Clin Pathol. 58(4):429-31. PMID: 15790713.
  • •  Immunodetection of SV40 large T antigen in human central nervous system tumours.
  • •  Radhakrishnan et al. 2004. J Virol. 78(13):7264-9. PMID: 15194802.
  • •  Intracellular approach for blocking JC virus gene expression by using RNA interference during viral infection.
  • •  Del Valle et al. 2004. J Virol. 78(7):3462-9. PMID: 15016869.
  • •  Primary central nervous system lymphoma expressing the human neurotropic polyomavirus, JC virus, genome.
  • •  Ricciardiello et al. 2003. Cancer Res. 63(21):7256-62. PMID: 14612521.
  • •  Induction of chromosomal instability in colonic cells by the human polyomavirus JC virus.
  • •  Del Valle et al. 2001. Cancer Res. 61(10):4287-93. PMID: 11358858.
  • •  Detection of JC virus DNA sequences and expression of the viral regulatory protein T-antigen in tumors of the central nervous system.
  • •  Masumori et al. 2001. Cancer Res. 61(5):2239-49. PMID: 11280793.
  • •  A probasin-large T antigen transgenic mouse line develops prostate adenocarcinoma and neuroendocrine carcinoma with metastatic potential.
  • •  Monoclonal antibodies specific for simian virus 40 tumor antigens.
  • •  Harlow et al. 1981. J Virol. 39(3):861-9. PMID: 6169844.