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Contributor Information

  • Name David Lane
  • Institute Cancer Research UK, London Research Institute: Clare Hall Laboratories
  • Primary citation Yewdell et al. 1986. J Virol. 59(2):444-52. PMID: 2426467.

Tool Details

  • Tool name: Anti-p53 [PAb242]
  • Alternate names: Antigen NY-CO-13, BCC7, Cellular tumor antigen p53, FLJ92943, LFS1, Mutant tumor protein 53, p53, p53 tumor suppressor, P53_HUMAN, Phosphoprotein p53, Tp53, Transformation related protein 53, TRP53, Tumor protein 53, Tumor protein p53, Tumor suppressor p53
  • Clone: PAb 242
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Mouse
  • Host: Mouse
  • Cancer type: Broadly Applicable
  • Molecular weight of the target: 53 kDa
  • Application: ELISA ; IHC ; IF ; IP ; WB
  • Strain: Balb/c
  • Description: Monoclonal antibody directed against p53, binding a distinct epitope in amino acids 18-27 at the N-terminus
  • Immunogen: SV40-transformed cell line BALB/c SVA31 E7
  • Immunogen UniProt ID: P04637
  • Isotype: IgG1
  • Research area: Apoptosis and autophagy; Cancer; Cell biology; Genetics
  • Myeloma used: Sp2/0-Ag14

  • For Research Use Only

Target Details

  • Target: p53
  • Target molecular weight: 53 kDa
  • Target background: Anti-p53 binds p53 protein, a crucial tumour suppressor protein, implicated in over 50% of cancers. p53 is a stress-regulated transcription factor that regulates cell cycle arrest and was first identified as an SV40 large T antigen-binding protein. p53 is involved in many mechanisms of anti-cancer function and plays a role in apoptosis, genomic stability, cell cycle regulation, and inhibition of angiogenesis. p53 is known as the guardian of the genome as it functions to prevent gene mutation. Mutants of p53 which frequently occur in human cancers, fail to recognise their DNA binding site, leading to the loss of tumour suppressor activity. p53 is typically found in low levels in healthy cells due to its short half-life, however, in disease states it is expressed in high amounts due to somatic mutations and is believed to contribute to transformation and malignancy. p53 mutations are found in over 50% of cancers with germline mutations, offering a predisposition to inherited cancer susceptibility syndrome. Anti-p53 Pab242 binds to an epitope within amino acids 18-27 at the N-terminus.

Application Details

  • Application: ELISA ; IHC ; IF ; IP ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: Store at -20° C frozen. Avoid repeated freeze / thaw cycles
  • Shipping conditions: Shipping at 4° C

Documentation

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References

  • •  Sabapathy et al. 2019. J Mol Cell Biol. 11(4):317-329. PMID: 30907951.
  • •  Eng et al. 2016. Protein Eng Des Sel. 29(1):11-21. PMID: 26508747.
  • •  Mayelzadeh et al. 2007. Oncogene. 26(21):2955-63. PMID: 17130840.
  • •  Lane et al. 1996. Oncogene. 12(11):2461-6. PMID: 8649788.
  • •  Yewdell et al. 1986. J Virol. 59(2):444-52. PMID: 2426467.