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Contributor Information

  • Name Jesper Svejstrup
  • Institute Absolute Antibody ; Cancer Research UK, London Research Institute: Clare Hall Laboratories

Tool Details

  • Tool name: Anti-RNA polII [CTD 4H8] rAb
  • Clone: CTD 4H8
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Recombinant
  • Conjugate: Unconjugated
  • Reactivity: Human ; Saccharomyces cerevisiae
  • Host: Mouse
  • Application: ChIP ; ELISA ; FACS ; IHC ; IF ; IP ; WB ; ChIP-seq
  • Description: RNAPII is an essential component of the RNAP II transcription elongation complex. Recruitment of RNA processing factors to the elongation complex is coordinated by the phosphorylation of Serine-2 and Serine-5 residues within the C-terminal repeat (YSPTSPS) domain of the largest subunit of RNAPII. Mutations that directly affect transcription by RNA polymerases rank among the most central mediators of malignant transformation. CTD4H8 can also be used in chromatin immunoprecipitation (ChIP) assays.
  • Immunogen: Peptide: 10 repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-Ser5
  • Isotype: IgG1
  • Research area: Genetics

  • For Research Use Only

Target Details

  • Target: RNA Polymerase II
  • Target background: RNAPII is an essential component of the RNAP II transcription elongation complex. Recruitment of RNA processing factors to the elongation complex is coordinated by the phosphorylation of Serine-2 and Serine-5 residues within the C-terminal repeat (YSPTSPS) domain of the largest subunit of RNAPII. Mutations that directly affect transcription by RNA polymerases rank among the most central mediators of malignant transformation. CTD4H8 can also be used in chromatin immunoprecipitation (ChIP) assays.

Application Details

  • Application: ChIP ; ELISA ; FACS ; IHC ; IF ; IP ; WB ; ChIP-seq

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Original hybridoma first published in: Kristjuhan et al. 2002. Mol Cell. 10(4):925-33. PMID: 12419235.