ANTIBODIES

Contributor Information
- Institute A*STAR Accelerate Technologies Pte Ltd
Tool Details
- Tool name: Anti-HPV16E2 [HPV16E2]
- Alternate names: Human papillomavirus type 16; Regulatory protein E2
- Clone: HPV16E2
- Tool type: Antibodies
- Tool sub-type: Primary antibody
- Class: Polyclonal
- Conjugate: Unconjugated
- Reactivity: Human
- Host: Rabbit
- Cancer type: Gynaecologic cancer
- Application: IHC ; WB
- Description: Cervical intraepithelial neoplasia (CIN) is caused by human papillomavirus (HPV) infection and is the precursor to cervical carcinoma. The completion of the HPV productive life cycle depends on the expression of viral proteins. Initiation of the viral productive replication requires expression of the E2 viral protein that cooperates with the E1 viral DNA helicase. A novel and important role for the HPV16-E2 protein in controlling host cell cycle during malignant transformation was recently revealed. Xells expressing HPV16-E2 in vitro were arrested in prophase alongside activation of a sustained DDR signal. In vivo, HPV16-E2 protein is present in cells that express both mitotic and DDR signals specifically in CIN3 lesions, immediate precursors of cancer, suggesting that E2 may be one of the drivers of genomic instability and carcinogenesis in vivo.
- Immunogen: GST-HPV16 E2 (209365)
- Immunogen UniProt ID: TBC
- Research area: Cancer; Cell biology; Microbiology
- For Research Use Only
Target Details
- Target: HPV16E2
- Target background: Cervical intraepithelial neoplasia (CIN) is caused by human papillomavirus (HPV) infection and is the precursor to cervical carcinoma. The completion of the HPV productive life cycle depends on the expression of viral proteins. Initiation of the viral productive replication requires expression of the E2 viral protein that cooperates with the E1 viral DNA helicase. A novel and important role for the HPV16-E2 protein in controlling host cell cycle during malignant transformation was recently revealed. Xells expressing HPV16-E2 in vitro were arrested in prophase alongside activation of a sustained DDR signal. In vivo, HPV16-E2 protein is present in cells that express both mitotic and DDR signals specifically in CIN3 lesions, immediate precursors of cancer, suggesting that E2 may be one of the drivers of genomic instability and carcinogenesis in vivo.
Application Details
- Application: IHC ; WB
Handling
- Format: Liquid
- Concentration: 0.9-1.1 mg/ml
- Storage buffer: Whole serum
- Storage conditions: -15ðC to -25ðC
- Shipping conditions: Shipping at 4ðC
References
- • Xue et al. 2015. Oncotarget. 6(33):34979-91. PMID: 26474276.
- • HPV16-E2 induces prophase arrest and activates the cellular DNA damage response in vitro and in precursor lesions of cervical carcinoma.
- • Xue et al. 2010. Cancer Res. 70(13):5316-25. PMID: 20530671.
- • HPV16 E2 is an immediate early marker of viral infection, preceding E7 expression in precursor structures of cervical carcinoma.