ANTIBODIES
Contributor Information
- Name Julian Gannon
- Institute Cancer Research UK, London Research Institute: Clare Hall Laboratories
Tool Details
- Tool name: Anti-Acetyl SMC3 [EM5.5.2]
- Clone: EM5.5.2
- Tool type: Antibodies
- Tool sub-type: Primary antibody
- Class: Monoclonal
- Conjugate: Unconjugated
- Reactivity: Xenopus laevis
- Host: Mouse
- Molecular weight of the target: 141 kDa
- Application: WB
- Description: SMC3 is a central component of cohesin, a complex required for chromosome cohesion during the cell cycle. The cohesin complex may form a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. Cohesion is coupled to DNA replication and is involved in DNA repair. The cohesin complex plays also an important role in spindle pole assembly during mitosis and in chromosomes movement.SMC3 is unacetylated in egg extracts. It is only acetylated in chromatin preparations from extracts where the DNA has replicated.
- Immunogen: Synthetic peptide - EEVSLRRVIGAKacetyl Kacetyl DQYFLDKK
- Research area: Cancer; Cell biology; Genetics
- For Research Use Only
Target Details
- Target: Acetyl SMC3
- Target molecular weight: 141 kDa
- Target background: SMC3 is a central component of cohesin, a complex required for chromosome cohesion during the cell cycle. The cohesin complex may form a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. Cohesion is coupled to DNA replication and is involved in DNA repair. The cohesin complex plays also an important role in spindle pole assembly during mitosis and in chromosomes movement.SMC3 is unacetylated in egg extracts. It is only acetylated in chromatin preparations from extracts where the DNA has replicated.
Application Details
- Application: WB
Handling
- Format: Liquid
- Concentration: 0.9-1.1 mg/ml
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15Ă°C to -25Ă°C
- Shipping conditions: Shipping at 4Ă°C
Documentation
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