ANTIBODIES

Contributor Information
- Name Shankar Balasubramanian ; John McCafferty ; David Tannahill ; Giulia Biffi
- Institute University of Cambridge
Tool Details
- Tool name: Anti-G-quadruplex [BG4]
- Clone: BG4 anti-DNA/RNA G-quadruplex antibody
- Tool type: Antibodies
- Tool sub-type: Primary Antibody
- Class: Recombinant
- Conjugate: Unconjugated
- Reactivity: Human
- Cancer type: Breast cancer
- Application: ChIP-seq ; ELISA ; IF
- Description: BG4 is an engineered, structure-specific antibody probe that binds with high selectivity and low nanomolar affinity to DNA G-quadruplex structures can can be employed to visualize DNA G-quadruplex structures in the genomic DNA of human cells. BG4 has high affinity for intramolecular and intermolecular DNA G-quadruplexes (Kd 0.5 ÄËĂÂĂÂ 1.6 nM, and 2.0 nM respectively) with no detectable binding to a RNA hairpin, single-stranded or double-stranded DNA.
- Immunogen: BG4, a single-chain antibody, was isolated from the Sanger phage display library through selection using a panel of intramolecular G-quadruplex structures.
- Research area: Genetics
- For Research Use Only
Target Details
- Target: DNA/RNA G-quadruplex structures in human cells
- Target background: BG4 is an engineered, structure-specific antibody probe that binds with high selectivity and low nanomolar affinity to DNA G-quadruplex structures can can be employed to visualize DNA G-quadruplex structures in the genomic DNA of human cells. BG4 has high affinity for intramolecular and intermolecular DNA G-quadruplexes (Kd 0.5 1.6 nM, and 2.0 nM respectively) with no detectable binding to a RNA hairpin, single-stranded or double-stranded DNA.
Application Details
- Application: ChIP-seq ; ELISA ; IF
Handling
- Shipping conditions: Shipping at 4ðC
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References
- • Biffi et al. 2013. Nat Chem. 5(3):182-6. PMID: 23422559.
- • Biffi et al. 2014. Nat Chem. 6(1):75-80. PMID: 24345950.
- • Yangyuoru et al. 2015. Angew Chem Weinheim Bergstr Ger. 127(3):924-927. PMID: 26300569.
- • HĤnsel-Hertsch et al. 2016. Nat Genet. :. PMID: 27618450.
- • G-quadruplex structures mark human regulatory chromatin.
- • Dual Binding of an Antibody and a Small Molecule Increases the Stability of TERRA G-Quadruplex.
- • Visualization and selective chemical targeting of RNA G-quadruplex structures in the cytoplasm of human cells.
- • Quantitative visualization of DNA G-quadruplex structures in human cells.