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Contributor Information

  • Name Steve Watson
  • Institute University of Birmingham

Tool Details

  • Tool name: Anti-CLEC2 [AYP2]
  • Clone: AYP2
  • Tool type: Antibodies
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human papilloma virus
  • Host: Mouse
  • Molecular weight of the target: 26 kDa
  • Application: WB
  • Description: Patients with rheumatoid arthritis, an inflammatory disease associated with increased microparticle production, have raised plasma levels of microparticles that expressed CLEC-2 but not GPVI. CLEC-2 can be used to monitor platelet-derived microparticles. The observation that microparticles derived from activated platelets retain CLEC-2 but lose GPVI highlights the potential use of measurement of surface expression of platelet receptors to screen for platelet activation in a wide variety of cardiovascular and inflammatory diseases.
  • Immunogen: A recombinant extracellular fragment of human CLEC-2 (aa 68-229), which is the extracellular domain of the protein.
  • Isotype: IgG1 kappa
  • Research area: Tissue-specific biology; Immunology
  • Myeloma used: Sp2/0-Ag14

  • For Research Use Only

Target Details

  • Target: C-type lectin-like receptor 2, CLEC1b
  • Target molecular weight: 26 kDa
  • Target background: Patients with rheumatoid arthritis, an inflammatory disease associated with increased microparticle production, have raised plasma levels of microparticles that expressed CLEC-2 but not GPVI. CLEC-2 can be used to monitor platelet-derived microparticles. The observation that microparticles derived from activated platelets retain CLEC-2 but lose GPVI highlights the potential use of measurement of surface expression of platelet receptors to screen for platelet activation in a wide variety of cardiovascular and inflammatory diseases.

Application Details

  • Application: WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: Hybridomas were cultured at 37? C and 96% relative humidity in air containing 6% CO2. Cells were grown in complete DMEM + Pen/strep + FCS was used at 10%. Cell density: Cells should be kept between 1-2x105 cells/ml and 5x105 cells/ml.
  • Storage conditions: 4°C
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Gitz E et al, Blood, 2014. PMID: 25150298