ANTIBODIES

Contributor Information
- Name Ayham Alnabulsi
- Institute Vertebrate Antibodies Limited
Tool Details
- Tool name: Anti-NSE [NSEP1]
- Clone: NSEP1
- Tool type: Antibodies
- Tool sub-type: Primary antibody
- Class: Monoclonal
- Conjugate: Unconjugated
- Reactivity: Human
- Host: Mouse
- Application: ELISA ; IHC ; WB
- Strain: Balb/c
- Description: Neuron specific enolase (NSE, or gamma-isozyme of enolase) is found at elevated concentrations in plasma in certain neoplasias, including paediatric neuroblastoma and small cell lung cancer.
- Immunogen: Ovalbumin-conjugated synthetic peptides corresponding to human NSE amino acid sequence:NSE-P1: aa's 416-433 - LGDEARFAGHNFRNPSVL
- Isotype: IgG1 kappa
- Research area: Cancer; Cell biology; Developmental biology; Metabolism; Neurobiology; Stem cell biology
- For Research Use Only
Target Details
- Target: Gamma-isozyme of human enolase (NSE)
- Target background: Neuron specific enolase (NSE, or gamma-isozyme of enolase) is found at elevated concentrations in plasma in certain neoplasias, including paediatric neuroblastoma and small cell lung cancer.
Application Details
- Application: ELISA ; IHC ; WB
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Storage buffer: PBS with 0.02% azide
- Storage conditions: -15ðC to -25ðC
- Shipping conditions: Shipping at 4ðC
References
- • Murray et al. 1993. J Clin Pathol. 46(11):993-6. PMID: 8254105.
- • Immunohistochemistry of neurone specific enolase with gamma subunit specific anti-peptide monoclonal antibodies.
- • Duncan et al. 1992. J Immunol Methods. 151(1-2):227-36. PMID: 1629611.
- • A simple enzyme-linked immunosorbent assay (ELISA) for the neuron-specific gamma isozyme of human enolase (NSE) using monoclonal antibodies raised against synthetic peptides corresponding to isozyme sequence differences.