ANTIBODIES

Contributor Information
- Name Ayham Alnabulsi
- Institute Vertebrate Antibodies Limited
Tool Details
- Tool name: Anti-RSV Nucleoprotein VP41 [4-18]
- Tool type: Antibodies
- Class: Monoclonal
- Conjugate: Unconjugated
- Reactivity: Virus
- Host: Mouse
- Application: ELISA ; IF ; WB
- Strain: Balb/c
- Description: Human Respiratory Syncytial Virus (RSV) is a major cause of lower respiratory tract illness and is the chief cause of hospitalization for respiratory tract illness in young children. There are two RS virus subgroups, A and B. The A and B subgroups circulate concurrently with subgroup A usually dominating. This antibody is useful for the diagnosis of RS virus subgroup A and B infections, since the nucleoprotein is very abundant within infected cells, which makes virus detection assays highly sensitive.The nucleoprotein is located in the nucleocapsid of the virion; it is abundant and forms a tight complex with virus genomic RNA. Anti-Nucleoprotein VP41 [RSV 4-18] reacts with both human RS virus Subgroups A and B.
- Immunogen: Gradient-purified RSN-2 virus (subgroup B) treated with 0.1% SDS at 100ðC for 2 mins.
- Isotype: IgG2a kappa
- Research area: Microbiology
- Myeloma used: P3X63Ag8.653
- For Research Use Only
Target Details
- Target: Human Respiratory Syncytial Virus (RSV) nucleoprotein VPN41
- Target background: Human Respiratory Syncytial Virus (RSV) is a major cause of lower respiratory tract illness and is the chief cause of hospitalization for respiratory tract illness in young children. There are two RS virus subgroups, A and B. The A and B subgroups circulate concurrently with subgroup A usually dominating. This antibody is useful for the diagnosis of RS virus subgroup A and B infections, since the nucleoprotein is very abundant within infected cells, which makes virus detection assays highly sensitive.The nucleoprotein is located in the nucleocapsid of the virion; it is abundant and forms a tight complex with virus genomic RNA. Anti-Nucleoprotein VP41 [RSV 4-18] reacts with both human RS virus Subgroups A and B.
Application Details
- Application: ELISA ; IF ; WB
Handling
- Format: Liquid
- Concentration: 1 mg/ml
- Storage buffer: DulbeccoĂÂs media containing 20% Fetal Bovine serum (DH20) prepared as follows (for final volume of 300ml: 237ml DMEM plus 60 ml Fetal Bovine Serum plus 3ml L-Glutamine).
- Storage conditions: -15ðC to -25ðC
- Shipping conditions: Shipping at 4ðC
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References
- • Gimenez HB et al. J Gen Virol. 1987 May
- • 68 ( Pt 5):1267-75. PMID: 3572364