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Contributor Information

  • Name Elena Klenova
  • Institute University of Essex

Tool Details

  • Tool name: Anti-BORIS [4A7]
  • Alternate names: Brother of the regulator of imprinted sites, Cancer/testis antigen 27, CCCTC binding factor (zinc finger protein) like, CCCTC-binding factor, CT27, CTCF paralog, CTCF T, CTCF-like protein, Ctcfl, CTCFL_HUMAN, dJ579F2.2, HMG 1L1, HMGB1L1, MGC163358, MGC16915, MGC16916
  • Clone: 4A7
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Mouse
  • Molecular weight of the target: 83 kDa
  • Application: ChIP ; ELISA ; IF ; IP ; WB
  • Description: Monoclonal antibody to understand the role of BORIS protein in prostate cancers.
  • Immunogen: The N-terminal domain (aa 2-257) of human BORIS (CTCFL) protein, expressed in E.coli
  • Immunogen UniProt ID: Q8NI51
  • Isotype: IgM kappa
  • Research area: Cancer; Genetics

  • For Research Use Only

Target Details

  • Target: BORIS
  • Target molecular weight: 83 kDa
  • Target background: BORIS protein is a transcriptional repressor encoded by CTCFL gene, and is normally present at high levels in testis. BORIS protein has been identified as Cancer-Testis Antigen (CTA) with testis-specific paralogue of the CCCTC-binding factor. Recent studies have demonstrated that BORIS is directly responsible for the transcriptional activation of TSP50 (testes-specific protease 50). BORIS protein is detected in all prostate cell lines and prostate tumours, but it is absent in benign prostatic hyperplasia tissues. Increased BORIS protein levels correlate with higher Gleason scores, T-stage and androgen receptor (AR) protein levels in prostate tumours. This reagent is for research and as a diagnostic tool.

Application Details

  • Application: ChIP ; ELISA ; IF ; IP ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: Store at -20°C frozen. Avoid repeated freeze / thaw cycles
  • Shipping conditions: Shipping at 4°C

Documentation

References

  •   Cheema et al. 2014. Prostate. 74(2):164-76. PMID: 24123052.
  •   Expression of the cancer-testis antigen BORIS correlates with prostate cancer.