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Contributor Information

  • Name Ian Waddell
  • Institute Cancer Research UK, Manchester Institute

Tool Details

  • Tool name: Anti-Tdp2 [Tdp2]
  • Clone: Tdp2
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Polyclonal
  • Conjugate: Unconjugated
  • Reactivity: Human
  • Host: Rabbit
  • Molecular weight of the target: 49 kDa
  • Application: WB
  • Description: DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 5'-phosphodiester bond, giving rise to DNA with a free 5' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase 2 (TOP2) active site tyrosine residue. Hydrolyzes 5'- phosphoglycolates on protruding 5' ends on DNA double-strand breaks (DSBs) due to DNA damage by radiation and free radicals. The 5'-tyrosyl DNA phosphodiesterase activity can enable the repair of TOP2-induced DSBs without the need for nuclease activity, creating a 'clean' DSB with 5'-phosphate termini that are ready for ligation. Has also 3'-tyrosyl DNA phosphodiesterase activity, but less efficiently and much slower than TDP1. May also act as a negative regulator of ETS1 and may inhibit nuclear factor-kappa-B activation.
  • Immunogen: Recombinant human protein with his tag
  • Research area: Cancer; Genetics

  • For Research Use Only

Target Details

  • Target: Tdp2
  • Target molecular weight: 49 kDa
  • Target background: DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 5'-phosphodiester bond, giving rise to DNA with a free 5' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase 2 (TOP2) active site tyrosine residue. Hydrolyzes 5'- phosphoglycolates on protruding 5' ends on DNA double-strand breaks (DSBs) due to DNA damage by radiation and free radicals. The 5'-tyrosyl DNA phosphodiesterase activity can enable the repair of TOP2-induced DSBs without the need for nuclease activity, creating a 'clean' DSB with 5'-phosphate termini that are ready for ligation. Has also 3'-tyrosyl DNA phosphodiesterase activity, but less efficiently and much slower than TDP1. May also act as a negative regulator of ETS1 and may inhibit nuclear factor-kappa-B activation.

Application Details

  • Application: WB

Handling

  • Format: Liquid
  • Concentration: 0.9-1.1 mg/ml
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

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References

  • •  Thomson et al. 2013. Anal Biochem. 436(2):145-50. PMID: 23416181.
  • •  Generation of assays and antibodies to facilitate the study of human 5'-tyrosyl DNA phosphodiesterase.