Product Image

Contributor Information

  • Name Alison Banham
  • Institute University of Oxford

Tool Details

  • Tool name: Anti-BCL11A [BCL11A/123]
  • Clone: BCL11A/123
  • Tool type: Antibodies
  • Tool sub-type: Primary antibody
  • Class: Monoclonal
  • Conjugate: Unconjugated
  • Reactivity: Human ; Rat
  • Host: Mouse
  • Molecular weight of the target: Predicted 91 kDa
  • Application: IHC ; IP ; WB
  • Description: The BCL11A/123 antibody specifically recognises the extra long form of the protein and not the long or short forms of BCL11A. The BCL11A/123 antibody is not cross reactive with the long form of the BCL11B protein that shares significant sequence identity with the immunogen.
  • Immunogen: C-terminus of extra long isoform of BCL11A, BCL11AXL (aa 637-835)
  • Isotype: IgG1
  • Research area: Cancer; Genetics; Neurobiology
  • Myeloma used: P3/NS1/1-Ag4.1

  • For Research Use Only

Target Details

  • Target: BCL11A (EVI9, CTIP1)
  • Target molecular weight: Predicted 91 kDa
  • Target background: BCL11A is a KrĂźppel zinc-finger transcription factor that was identified originally from a chromosome translocation in aggressive B-cell chronic lymphocytic leukaemia. The gene may function as both an oncogene and a tumour suppressor. Alternative splicing generates multiple isoforms, the BCL11AXL being the largest and most abundant transcript. The protein is differentially expressed during B-cell development and is strongly expressed in plasmacytoid dendritic cells.

Application Details

  • Application: IHC ; IP ; WB

Handling

  • Format: Liquid
  • Concentration: 1 mg/ml
  • Storage buffer: PBS with 0.02% azide
  • Storage conditions: -15°C to -25°C
  • Shipping conditions: Shipping at 4°C

Documentation

References

  •   Chetaille et al. 2009. Blood. 113(12):2765-3775. PMID: 19096012.
  •   Molecular profiling of classical Hodgkin lymphoma tissues uncovers variations in the tumor microenvironment and correlations with EBV infection and outcome.
  •   Marafioti et al. 2008. Blood. 111(7):3778-92. PMID: 18218851.
  •   Novel markers of normal and neoplastic human plasmacytoid dendritic cells.
  •   Weniger et al. 2006. Leukemia. 20(10):1880-2. PMID: 16871282.
  •   Pulford et al. 2006. Leukemia. 20(8):1439-41. PMID: 16710303.
  •   Gains of the proto-oncogene BCL11A and nuclear accumulation of BCL11A(XL) protein are frequent in primary mediastinal B-cell lymphoma.
  •   The BCL11AXL transcription factor: its distribution in normal and malignant tissues and use as a marker for plasmacytoid dendritic cells.
  •   Liu et al. 2006. Mol Cancer. 5:18. PMID: 16704730.
  •   Fn studies of BCL11A: characterization of the conserved BCL11A-XL splice variant and its interaction with BCL6 in nuclear paraspeckles of germinal center B cells.